Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,383,960	C→T	Q103Q (CAG→CAA)	mdh ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,383,960	0	C	T	100.0%	51.5 / NA	19	Q103Q (CAG→CAA)	mdh	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

TAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGG  >  NC_000913/3383728‑3384196                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             tAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTAGCCTGGCTGTTTGCCTTTAAGTTCCGCAAAAAAGGTGTTGGAACGAATGCTATCCAGCGTGGTAACGACGAACAGATTGTTTTTGTCATACACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAg                                                                                                                                                                                                                                      >  1:91868/1‑241 (MQ=255)    gtgaatagtgttgcgtttgcgccGGCACTTCAACTGCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGGTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATg                                                                                                                                                                                                                         <  1:217429/230‑1 (MQ=255)                        aCCGGCAANTNNNCNNCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGGGTTGGAANGAATGATATCCAGCGNGGNAACGNCGANCAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCANGTTNTCGCCNNTNGTTGTANNNgg                                                                                                                                                                                                                                     >  2:147833/1‑219 (MQ=255)                         ccGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAgg                                                                                                                                                                                                    <  1:103731/251‑1 (MQ=255)                                                         ttCAGTTCCGCAACAAAGGTGTTGGAACGAATGATAGCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCAGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGTGGAGGTTTTCGCAGCTTTTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGAt                                                                                                                                                                                          <  1:219183/229‑1 (MQ=255)                                                                                      aaTGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATACTACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGACGGCGTTAACGTTAAACAGGTCGGAACGATACATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTgcgc                                                                                                                                       >  1:126303/1‑251 (MQ=255)                                                                                               cAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTAAGCACTTCAGCCGCCATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTcc                                                                                                                                  >  1:149598/1‑247 (MQ=255)                                                                                                        aaCGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCg                                                                                                                     <  1:80357/251‑1 (MQ=255)                                                                                                                             ntCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATT‑CAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCAGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCttt                                                                                               >  1:134606/2‑251 (MQ=255)                                                                                                                                       ccGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCa                                                                                                             >  1:47970/1‑228 (MQ=255)                                                                                                                                         ggCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACAGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCGTTCCAGCGCCGTAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcag                                                                                    <  1:194228/251‑1 (MQ=255)                                                                                                                                                                                    aCCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGCTTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCAc                                         >  1:74455/1‑251 (MQ=255)                                                                                                                                                                                        ggTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACAAGGTACTTCACGATGCAGGAGTTACCGATAACCAGGTGGGAACGCTCCATACCCGGTTTACGCGCCACGCCTGCAGACATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACCCCGGGAGTCACTgg                                      >  1:220250/1‑250 (MQ=255)                                                                                                                                                                                                 taataCCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGTTCGGAACGATCCATACCCGGTTGACGCGCTACGCCTGCAGAGATAAGAACGATATCTgc                                                                                                                                         <  1:185142/142‑1 (MQ=255)                                                                                                                                                                                                      ccAATGCGCGCGTTCGGGCAGGTTTTCGCAACTTTTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTGCACCAGAAAAACCTGTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACan                       <  1:23455/251‑2 (MQ=255)                                                                                                                                                                                                                  tgcGGGCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACTCTCTATACCCGGTTTACGCGCCACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAGCCTGTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTg                                       <  1:212962/221‑1 (MQ=255)                                                                                                                                                                                                                       gCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCg                                                                                                                                                                                                                      <  1:5382/43‑1 (MQ=255)                                                                                                                                                                                                                       gCAGGTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATGCCg                                                                                                                                                                                                                      >  2:5382/1‑43 (MQ=255)                                                                                                                                                                                                                           gTTTTCGCAACTTGTTGTACCAGGTTTTTCACGATTCCGGCGTTAACGTTAAACAGGTCGTAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGTCTCAGATCGACAGCCACACCGGGAGTCACTGGATCGATATCATACAGAGAGAGTTCTGAACCTGAAgt  <  1:147833/251‑2 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             TAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGG  >  NC_000913/3383728‑3384196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑