Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,635	G→A	E177K (GAA→AAA)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,635	0	G	A	100.0%	15.3 / NA	7	E177K (GAA→AAA)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCG‑GTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCT  >  NC_000913/3211436‑3211840                                                                                                                                                                                                        |                                                                                                                                                                                                               ccATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCC‑GGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAAAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAggg                                                                                                                                                            >  1:62197/1‑251 (MQ=255)                gCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACACCAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGACTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAAAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATc                                                                                                                                              >  1:91896/1‑251 (MQ=255)                       cAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAAAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTcn                                                                                                                                        <  1:44730/250‑2 (MQ=255)                                                                                                                           gggTTATCGCTCTGTTTGCGTTTGGTTTGGCGCCCCCCGGATGGGACAACGGCGTGAAGCGTTTTGGTGGAAACCCAAAAAACCGCAAGTCATGGATGGATGCGGGTATGTGGGTCTCTACCGATCAGGACCGCGGTTGCTATCGTTTCCGCGAGCGTGTGATTTTCCCCATCCGCAATAAACGCGTGT‑GGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACAATGCCCCAAAATACCGGAACTCGCCg                                  <  1:92176/250‑1 (MQ=255)                                                                                                                                      cgctTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAAAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCG‑GTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGan                         <  1:53503/249‑2 (MQ=255)                                                                                                                                                   nttttGCGCCCCCCGGTTGGGACAACGTCCTGAAGCGGTTTTGCGGCAATCCAAAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCG‑GTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGaagaccggaagagcaa           >  1:135436/2‑235 (MQ=255)                                                                                                                                                           ccccccGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAAAAAATCGCCAGTCCTTGATTGCTGAGGGGCTGTTGGTCACTCACGATCAGGGCCGCAGTTACGATCGTTTCAGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCG‑GTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATAACTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCt  >  1:190751/1‑251 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                               CCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCG‑GTCGGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCATAAAGGCCGCCAGCT  >  NC_000913/3211436‑3211840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑