Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,674	G→A	intergenic (+149/+47)	yqiK → / ← sibD	flotillin family inner membrane protein YqiK/small RNA SibD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,674	0	G	A	100.0%	30.9 / NA	15	intergenic (+149/+47)	yqiK/sibD	flotillin family inner membrane protein YqiK/small RNA SibD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

CGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTT‑CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTA  >  NC_000913/3194450‑3194913                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ngTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTT‑CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTgg                                                                                                                                                                                                                                           >  1:130673/2‑231 (MQ=255)                        cATTGACTTCACCCTTAACATCAACCACTCCCGTCGAAGCACAAGCAGCGTCAACCCTCTTACCAG‑AATACTCACTAGCCCAACAGGCCGGGGATTTTTTATATCTGCGTTCCGCTACAAAGTGCAACTGATCCGGTTGTTGCAGCATTCTGCGTGACGGCTCGCGGGAGGCACAACTGCATCAGAGAAAGCATTGCGTGAAGTCTGGCGCTCATCAACAACCGGGGCGTCAGCAGGGGGCTGAAACggg                                                                                                                                                                                                 >  1:114527/1‑250 (MQ=19)                                   cccTTAACCTCAACACCTCCCGTAGAAGAAAAAGCAGAGTACACCCTCTT‑CCAGGAATAATCCCTGGCCCACACAACCGGGCATTTTTTATATCTGCGTTCCGCTAAAAAGCGCCAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGACGGCCAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCCGCAggcgccggtaaagcggttgatctgaa                                                                                                                                                                                      >  1:158998/1‑226 (MQ=25)                                                                              cccTCGT‑CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTGTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGTTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAggggg                                                                                                                                                                                                           <  1:11280/186‑1 (MQ=255)                                                                                   nnnnnnggNNNNNTCCNTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTgg                                                                                                                                                                                                                                           <  2:130673/144‑1 (MQ=21)                                                                                                       cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTCAAAAGTGCAAATGATCCGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGACGGCCAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTgttgtt                                                                                                                   >  1:29453/1‑250 (MQ=255)                                                                                                       cccAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTgttgtt                                                                                                                   >  1:5104/1‑250 (MQ=255)                                                                                                                                   aggccatcagccggggatcAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTAGGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAg                                                                                       <  1:203888/232‑1 (MQ=25)                                                                                                                                                aaGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGTGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCGTATTAACAGCCAATCTGAggggg                                                                         <  1:61944/251‑1 (MQ=255)                                                                                                                                                                      ncagcaTTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAgggggcacctgccagggtgctggcgct                                                   >  1:175246/2‑229 (MQ=25)                                                                                                                                                                          caTTTTGCGTGACGGCTAGGGGAAGGCAACACTGCATCAGAGAAAGCATTGCGTGGAGGCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAAt                                                                  >  1:65150/1‑232 (MQ=255)                                                                                                                                                                                               gAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGt                                                  <  1:84485/227‑1 (MQ=255)                                                                                                                                                                                                    cAAAACTGCAT‑AGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCt                                                                                                <  1:125153/175‑1 (MQ=255)                                                                                                                                                                                                         cTGCATCAGAGAAAGCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCgc                                                                                                                                                                                   >  1:29035/1‑87 (MQ=255)                                                                                                                                                                                                                       gCATTGCGTGGAGTCTGGCGCTCATCAACAATCGGGGCGTCCGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGCAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTT‑ACTTTCGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTa  >  1:92329/1‑250 (MQ=25)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                CGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTT‑CCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCATCAACAATCGGGGAGTCAGCAGGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGGCCTTACAGAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGATTGTGTTGTTACTCGTAAGTTTCGCAGCTTATTAACAGCCAATCAGAGGGGGGAGAAATCCTCCCTCACCCTTGTTCCTTTACTTTAGGTTGAAAAAAAATCAGCGTCAAGAAGGTTTCATTA  >  NC_000913/3194450‑3194913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑