Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,822	G→A	Q91Q (CAG→CAA)	zupT →	divalent metal ion transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,822	0	G	A	100.0%	26.8 / NA	11	Q91Q (CAG→CAA)	zupT	divalent metal ion transporter ZupT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

CGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGC  >  NC_000913/3182603‑3183053                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        cgcgTTTCTCGGCTTTCTCGGGCAAAAACCCTCGAACCGCTTACTGTCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAAGGCTTCCTGCCGCACTATCAGCGTAAGGAATGTCGCTTGTGTTGGTTTATGGATTGTTTATCGTCGGTCTTCTTGGCTATTTTGGTGTTGACCTCATGTTGCCACAGGCTTATCCGCAAGATTTAATGCAAAAATCGGTGCATCCGTTGc                                                                                                                                                                                                          <  1:126674/251‑1 (MQ=255)                                                                                                                          gcTGAAGGAATGTCGCCTGTGTTGGGTTATGTAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGATCGCATGTTGCCACATGCTCATCCGCAAGATTGAATGCAAAAATCGGTTCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCTCTCTCGCCATCAGTCTGCATAACTGCCCGGAAGTGATGGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGc                                                                                 <  1:158399/251‑1 (MQ=255)                                                                                                                          gcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGc                                                                                 <  1:158394/251‑1 (MQ=255)                                                                                                                                         ccTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCAGCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAgc                                                                                                                                                                                           <  1:117924/130‑1 (MQ=255)                                                                                                                                         ccTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGAATGTTGCCACATGCTCATCCGCAAGATTTAATGCAAAAATCGGTGTAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAGGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGtt                                                                  >  1:91376/1‑251 (MQ=255)                                                                                                                                                                           tGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCANAACNNNNNggnn                                                                                                                                         >  2:135183/1‑144 (MQ=255)                                                                                                                                                                                          gCCTGGACNNCANNTNGNCACCTNCTCATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                                                                                                                                                                          >  2:138624/1‑66 (MQ=255)                                                                                                                                                                                          gCCTGGACCGCATGTTGCCACATGCGTATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                                                                                                                                                                          <  1:138624/66‑1 (MQ=255)                                                                                                                                                                                                          gCCACATGCTCATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACgn  <  1:137243/250‑2 (MQ=255)                                                                                                                                                                                                          gCCACATANTNNNCNNCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATNAAGCGCACAGGCATTNTGNt                                                                                                                                                                           >  2:155341/1‑81 (MQ=255)                                                                                                                                                                                                           ccACATGTTCATCCGCAAGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGAACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTc         >  1:49440/1‑242 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        CGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGC  >  NC_000913/3182603‑3183053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑