Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,069	G→A	G122G (GGC→GGT)	ygiD ←	4,5‑DOPA dioxygenase extradiol

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,069	0	G	A	100.0%	20.5 / NA	8	G122G (GGC→GGT)	ygiD	4,5‑DOPA dioxygenase extradiol
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

CCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGC  >  NC_000913/3181855‑3182300                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        cccACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGAGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTc                                                                                                                                                                                                     >  1:162571/1‑251 (MQ=255)                       aNCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCg                                                                                                                                                                                                                                      >  1:171115/1‑196 (MQ=255)                                                  aTGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCGTGGTCAAAGc                                                                                                                                                                                                                            <  1:5575/179‑1 (MQ=255)                                                                         ccAACATTATTCCTTCATCTCGCAGCGCTGCCCGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGt                                                                                                                              >  1:214070/1‑250 (MQ=255)                                                                                             cGCAGCGCTGCCAGTTTGCGCCCCATTTCGATATGCCAGGCTGCAGGGTGGCGTCTGTCGAGACTCAACAGCACCATCCGGATATCAGCGGCAGGATACATCTGAATCTGCACGCCCCCCGAACCGTGGGCAAAGCCCCAGGcttctt                                                                                                                                                                                                                <  1:201254/148‑1 (MQ=255)                                                                                                                                        cAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTc                                                              <  1:51473/251‑1 (MQ=255)                                                                                                                                        cAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAACCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTc                                                              >  1:93453/1‑251 (MQ=255)                                                                                                                                                                                                     aaTCAGCACGCCCCACGAACCGTGGTCAAAGCCCCAGGCTGCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGACGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCCCCAAAGTCATGAATCTTGGGCGGCGTCTCCAGCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGACGAAACCACCACAATCGCTTGCGGGCGTGGc  <  1:216826/250‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        CCCACGGATACGGTGAACTATCACCGTGCCACTTCACTGTGCGCAGGTTATGCACCACGTTACCGCTGGCGACCAACATTATTCCTTCATCTCGCAGCGCTGCCAGTTTGCGCCCCATTTCGAAATGCCAGGCGGCAGGTTTGCTACTGTCGATACTCAACTGCACCATCGGGATATCAGCGTCAGGATACATCTTAATCAGCACGCCCCACGAGCCGTGGTCAAAGCCCCAGGCTTCTTTATCCAGCGTCACCGGGATCGGCGCTAACAGCTCAACCAGACGCTGTGCCAGCGCAGGCGAACCCGGAGCAGGATAATGCGTATCGTACAGCGCCTGCGGGAAGCCACCAAAGTCATGAATCGTGGGCGGCGTCTCCATCGCGGTCACTCCTGTTCCACGGGTAAACCAGTGAGCCGAAACCACCACAATCGCTTGCGGGCGTGGC  >  NC_000913/3181855‑3182300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑