Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,260	G→A	D7D (GAC→GAT)	yqiB ←	DUF1249 domain‑containing protein YqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,260	0	G	A	100.0%	26.2 / NA	11	D7D (GAC→GAT)	yqiB	DUF1249 domain‑containing protein YqiB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

TGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGAC  >  NC_000913/3177101‑3177488                                                                                                                                                                |                                                                                                                                                                                                                                     ggTCACCAGGGTAGTGTATCGGTTCGATTCCACAATCGGCAGCCTATATTGTGCGTTTGCCACCGGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTGAGGAAAAGCAGGTATGTAACGCTTCATTGATGCCCACTCATTTATTAACGCTTGATTAGGCAGCTGCAGCCAGTGCAAATCGATGACCGACGCTGCg                                                                                                                                            <  1:139962/249‑1 (MQ=255)                      ggTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAgg                                                                                                                                                                                                                                 >  1:12976/1‑144 (MQ=255)                                      gTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGCGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTAccc                                                                                                                   >  1:122899/1‑238 (MQ=255)                                            cGATATTGTGCGTTTGCCGCCTTATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTTATGATGCAGCTGCGGCCATTGCAAAGCGATGACCGACTCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTcccn                                                                                                <  1:20304/251‑2 (MQ=255)                                                                                   ccGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTGAACACTTGATGATGCAGCTTCAGCCATGTCAAAGCGATGACCGACGCTGCGTGGTCGATTCTCCCCGCTTCTACCCACGGGTAGCCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGc                                                         <  1:162488/251‑1 (MQ=255)                                                                                          cGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGtttt                                                                >  1:14024/1‑237 (MQ=255)                                                                                                 ncgcgGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGACTATCTTCGTTTTCATCAGCCAGACCGTGACTAc                                           >  1:133760/2‑251 (MQ=255)                                                                                                                   cAATGGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTGATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCg                                                                                                                                      <  1:110148/142‑1 (MQ=255)                                                                                                                            aaaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATGTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTGTCCCCTCTTCTACCCACTGTTATGcc                                                                                                        <  1:173645/163‑1 (MQ=255)                                                                                                                                      nnnnnnaGNNNNNTCANTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTAt                                                                                                           <  2:132861/144‑1 (MQ=255)                                                                                                                                          acaGGCGCATCATTTCAGGAAAATCAGGTGTGTAACGCTTCATTTATGCCCACTCAGTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTTCCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGan  <  1:137340/251‑2 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                                     TGTCACCAGGGTAGTGTATCGGGTCGATTCCACAATCGTCAGCCGATATTGTGCGTTTGCCACCTGATAGCTTACAGTTTCGCCGGGTGCGTCATTGCGCGGTAACAAACGGCGCAATTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGAC  >  NC_000913/3177101‑3177488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑