Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,160,223	G→A	A308A (GCC→GCT)	ygiQ ←	radical SAM superfamily protein YgiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,160,223	0	G	A	100.0%	26.1 / NA	12	A308A (GCC→GCT)	ygiQ	radical SAM superfamily protein YgiQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

GCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAC  >  NC_000913/3159997‑3160380                                                                                                                                                                                                                                   |                                                                                                                                                              gCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTGGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTc                                                                                                                                       <  1:195751/251‑1 (MQ=255)                                             cGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTGTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACgg                                                                                           <  1:193939/251‑1 (MQ=255)                                                                    tgGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCACTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGGTGATCCCCACATAGCGGTCGCCGTGTTTTTCAATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGAAGACTACGCGAAGCATGAGCGTACAGAACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGGGGCTGCACgg                                                                    >  1:95955/1‑251 (MQ=255)                                                                                     ncAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTcc                                                   >  1:25075/2‑251 (MQ=255)                                                                                        acgcAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGCTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGCGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGACGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGGGGCTGCACGGTTACgg                                                              >  1:109121/2‑237 (MQ=255)                                                                                                     ntCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCg                                                                                      >  1:23338/2‑200 (MQ=255)                                                                                                                         gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTTCCACGGTTTCGGGCGCGGGGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTgn                          <  1:22596/240‑2 (MQ=255)                                                                                                                                      cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTGTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAc  <  1:96126/251‑1 (MQ=255)                                                                                                                                      cGGGGTGATCCACACAGAGCGGTCGCCGGGTTTTTGCATCAATGCGCGGGCACAGCCAGGGTGGGTTTCGGGGTGCCGAATACGCGAAGCATGATCTTACAGCACGTTAGCGCCCTTCACTTGCTCGAAAGAAGTCAGCAACACTGAGGTGTTTTCCCACGGTTGCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCt                                               <  1:222683/206‑1 (MQ=255)                                                                                                                                                                                                  ggtggttTCGTGGTGCAGAATACGCGAAGCATGATCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTcc                                                                                                <  1:159626/95‑1 (MQ=255)                                                                                                                                                                                                           gtggtgCANANNNCNNGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAANGAAGGCAGCAACACGNAGNTTTTNTCCNACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTgg                                                        >  2:148806/1‑128 (MQ=255)                                                                                                                                                                                                           gtggtgCAGAATACGCGAAGCATGAGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTgg                                                        <  1:148806/128‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                              GCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATCTTCAC  >  NC_000913/3159997‑3160380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑