Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,147,042	G→A	intergenic (‑305/+555)	yghW ← / ← yqhI	DUF2623 domain‑containing protein YghW/protein YqhI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,147,042	0	G	A	100.0%	23.9 / NA	11	intergenic (‑305/+555)	yghW/yqhI	DUF2623 domain‑containing protein YghW/protein YqhI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

TTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCGGATAGC  >  NC_000913/3146813‑3147229                                                                                                                                                                                                                                      |                                                                                                                                                                                            tttCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCAGAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTGGATTCGAGTGTCCAGTTCCGCGAAGtn                                                                                                                                                                         <  1:54407/250‑2 (MQ=255)                                                       atTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTgacgacn                                                                                                                   <  1:53898/250‑2 (MQ=255)                                                              gAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGCATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACg                                                                                                           >  1:221946/1‑251 (MQ=255)                                                               aaCCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTAGTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTc                                                                                                                                                                                  <  1:197522/179‑1 (MQ=255)                                                                                  gCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGgcgn                                                                                                                                                          <  1:44786/184‑2 (MQ=255)                                                                                           gATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTGATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATAcgcg                                                                              <  1:66319/251‑1 (MQ=255)                                                                                                                   ggggCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTAGTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTcc                                                                                                                                                                                       <  1:141512/122‑1 (MQ=255)                                                                                                                                                                tataCNNATNNNAANNCTTATTATTGGCTTTCAACGCCGNCTCGTAAGCAGGCCAGCCATCGTTGATTCNANTNTCCANNTCCNCGACGTGGAGNAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGANCNTAAAAAGGCCCCGCGCAGGCCCGTTCCGGATNCGCGACAGCCGCCGCGTTc                                                               >  2:157336/1‑197 (MQ=255)                                                                                                                                                                tagaCGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCTTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCTGTTCCGCTAAGTGGAGGAGTAAAGGCGCTTCAAGCTTCGCCACAGCGGCAGTGGGTGCCTGACGACCATAAAACGGCTCCGTGCAGGCCAGTTCCGGATACGCGTCAGCCGCCGCGTTc                                                               <  1:157336/194‑1 (MQ=255)                                                                                                                                                                       cTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCGGATAGc  >  1:89416/1‑251 (MQ=255)                                                                                                                                                                                                 aCGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGAGTGTCCAGTTCCGCGAAGTGGCGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCAc                         >  1:91897/1‑202 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                            TTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGCAGATTTGTCATAACGGGGCGTGGAATCATTATGGAATCCGTGATTAACCCCCGGATAGATATACGCCTCATAAACCTTATTATTGGCTTTCAACGCCGCCTCGTAAGCAGGCCAGCCCTCGTTGATTCGGGTGTCCAGTTCCGCGAAGTGGAGTAGTAAAGGCGCTTCAATCTTCGCCACATCGGCAGTGGGTGCCTGACGACCATAAAACGGCACCGCGCAGGCCAGTTCCGGATACGCGACAGCCGCCGCGTTCGATACGCCACCGCCATAGCAAAATCCGGTAATACCCACTTTGCCTGTCGCTTGCGGATAGC  >  NC_000913/3146813‑3147229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑