Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,118,513	G→A	R200R (CGC→CGT)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,118,513	0	G	A	100.0%	15.3 / NA	7	R200R (CGC→CGT)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

GCCGACACGAAGGAAGCGTTCAGATCCGGTTTTGTTCCCGGCGTGGTGACGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCG  >  NC_000913/3118337‑3118753                                                                                                                                                                                 |                                                                                                                                                                                                                                                 nccGACACGACGGAAGCGTTCAGATCCGGTTTTGTTCCCGGCGTGGTGACGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAACGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGt                                                                                                                                                                        >  1:56772/2‑251 (MQ=255)                                                       nnnnnnggNNNNNTATNAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACACTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAACGGTTACTCTCGATCCCCGAGGAGAAc                                                                                                                                                                                                                       <  2:129839/144‑1 (MQ=255)                                                                  aGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAACGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTTTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGAAGCCCGCCAGTTCTTGCGCGTCCTCAAGGc                                                                                                       <  1:158966/251‑1 (MQ=255)                                                                                                                                 tttGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAACGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTg                                         <  1:78170/250‑1 (MQ=255)                                                                                                                                                    aGATCGATTTGCTTATACAGCGAGTCGAAACGTTTACTCTCGATCACCGAGGAGAACGTAAGACAACCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCCTTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCACCCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGc                     >  1:191788/1‑251 (MQ=255)                                                                                                                                                                      aGCGAGTCGAAACGTTTACTCTCGATCACCGCCGAGAACGTCAGACAAACCTGCTCTGTATTAGCCGGACCGCTGTTCCTGGCCGTTACCCGCGAAACCGCCTTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                                                                                                          >  1:84955/1‑132 (MQ=255)                                                                                                                                                                       gctaGTCGAAACGTTCACTCTCGATCACCGTGGAGAACGTAAGACAACCCTGTTCTGTATTCTCCGGACAGCTGTTACTGGACGTTACCGTCAAACCCGCATTGCTTTTCTGGGCATTGGTGCCCGCTAGTTCTTGCGCGTCCGCAAGGCTAAAGGACGCTTTTTCACCCGCACGCAAGCTACGCGCAGTGTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCg  <  1:114506/248‑1 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GCCGACACGAAGGAAGCGTTCAGATCCGGTTTTGTTCCCGGCGTGGTGACGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCG  >  NC_000913/3118337‑3118753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑