Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,589	G→A	G53S (GGC→AGC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,589	0	G	A	100.0%	27.4 / NA	12	G53S (GGC→AGC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

ATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATC  >  NC_000913/3104365‑3104784                                                                                                                                                                                                                                 |                                                                                                                                                                                                    ataaaaaCAGTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGAGGAAAAAATATCTCGCGCTGGCTTGGATTGCGCCGTGGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTGTGATATTCGGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGt                                                                                                                                                                           <  1:113082/251‑1 (MQ=255)          gTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACAAAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATATCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACAAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGAc                                                                                                                                                                  >  1:81394/1‑251 (MQ=255)                          ccGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCg                                                                                                                                                  <  1:15314/251‑1 (MQ=255)                            ggCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAACTATCTCGCGCTGGCTTTGATTGCGCCGTTGATCATCTCCTGTTCGACGACCAAAAACGGAGATACCTCTAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCAACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGACATACACCGAt                                                                                                                                                >  1:102119/1‑251 (MQ=255)                                                                                       ctgtcTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCAt                                                                                     >  1:168537/5‑251 (MQ=255)                                                                                                                              acCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCATCGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATc                                                                                                                                         <  1:15849/160‑1 (MQ=255)                                                                                                                               ccAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCtt                                                                                                                                                                                                 >  1:69664/1‑103 (MQ=255)                                                                                                                                              ncTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCACAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTg                              >  1:39499/2‑251 (MQ=255)                                                                                                                                                                      aTNNNNNCGGNTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACCCCGATCAATATCAGACCCGCAGCCACATCAACATCg                                                                                                                 <  2:135649/144‑1 (MQ=255)                                                                                                                                                                         ccAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCATCAAAGAGGTGGTGATCGCTGGACCTAAGGCCTACGTGAAATACACCGATCAATATCAGACCAGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGACGGGCAATTATCAAACCGTGATTGATGGGTGACGATCCGAGTTCGGTCGAt   >  1:154000/1‑251 (MQ=255)                                                                                                                                                                                            gATGGGGCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCNNTCAatann                                                                                                                                         >  2:4471/1‑96 (MQ=255)                                                                                                                                                                                                 ggCAATTTGCCCACAATATTGAGAACATCTGGAGCTTCAAAGCGGTGGTGCTCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAGCCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGAtc  >  1:94467/1‑228 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                    ATAAAAACAGTGCCGGAGCACGCCTCCGGCAACTTGCATAAAAACAAACACAACACGCACCCGGAATGATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACCAAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTGATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATC  >  NC_000913/3104365‑3104784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑