Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,779	G→A	intergenic (‑51/+125)	ansB ← / ← yggN	L‑asparaginase 2/DUF2884 domain‑containing protein YggN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,779	0	G	A	100.0%	27.7 / NA	12	intergenic (‑51/+125)	ansB/yggN	L‑asparaginase 2/DUF2884 domain‑containing protein YggN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

GGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATGGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGC  >  NC_000913/3100600‑3101000                                                                                                                                                                                    |                                                                                                                                                                                                                              ggTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTACAACCCATAACCAGTGCGGCACGTGCCGTCTTTTTGAAACACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAACCAAttt                                                                                                                                                                                                        >  1:187083/1‑203 (MQ=255)  gTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAAAGCTGCACCACTAAAACACATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACa                                                                                                                                                        >  1:120995/1‑250 (MQ=255)      cGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTACAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGACAAACTCCCTTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAACTTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACg                                                                                                                                                   >  1:162024/1‑251 (MQ=255)                       gCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGGAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCAt                                                                                                                                   >  1:180444/1‑250 (MQ=255)                                          ntAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAg                                                                                                                                                                        >  1:63642/2‑194 (MQ=255)                                                    gATATTGGATAATGCCAATGCTGCACCACGAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACGCCATTTCATGCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAAc                                                                                                                                                                                                   <  1:219879/157‑1 (MQ=255)                                                                  ccAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACaaan                                                                                                                                                                        <  1:39517/170‑2 (MQ=255)                                                                                           aaCCAGTGCGGCAAGTGCCGTCGTTTTGAAAAACTCCATTTCATTCCTCCATTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACATGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTTCGGCTATc                                                               <  1:169569/250‑1 (MQ=255)                                                                                                     gCAAGTGACGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGCGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGAcc                                                                                >  1:166899/1‑223 (MQ=255)                                                                                                            ccGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACCAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAAcccg                                             >  1:77135/1‑248 (MQ=255)                                                                                                                        aaaaCTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCgg                                            <  1:197469/240‑1 (MQ=255)                                                                                                                                                       aCGCTACGCATTATCCCTTAGCTCTGTATAGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTgn  <  1:41697/251‑2 (MQ=255)                                                                                                                                                                              ntGTATNGGAANTTTGACGTTAAACAANTTACAACGGGAATATAGTANNGNGATCTNCAAAg                                                                                                                                                                        <  2:84699/61‑1 (MQ=255)                                                                                                                                                                                    |                                                                                                                                                                                                                              GGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCATTATCCCTTAGCTCTGTATGGGAAATTTGACGTTAAACAATTTACAACGTGAATATATTTTGGAGATCTACAAAGTTAGAGGCAGGTAACAAAACGAAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGC  >  NC_000913/3100600‑3101000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑