Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,689 G→A E136K (GAA→AAA)  galP → galactose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,6890GA90.9% 26.6 / NA 11E136K (GAA→AAA) galPgalactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/7);  minor base C (1/0);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGGAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACAC  >  NC_000913/3088496‑3088933
                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
cAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGACTGATGATCGGCGCAATATTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCCAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATAACTATCGGGATcc                                                                                                                                                                                             >  1:141130/1‑251 (MQ=255)
                                                    nnnnnnnnnnttnnnnNCGGNTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAATTCGTg                                                                                                                                                                                                                                          <  2:133938/144‑1 (MQ=255)
                                                                           gCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGGTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAAGTCGTGGCAGTAGGATCGCGATGTATCAGTTGATGATCACGAGCGGGATCCTCGGTGCTTATCTGTCTGATGCCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATcc                                                                                                                  <  1:100195/251‑1 (MQ=255)
                                                                                 ctctGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGAGCCGAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTCAACCGGTGCCTGGCGCTGGATGCTGGGGGTGATTATCATCCCGGCa                                                                                                             >  1:85062/1‑250 (MQ=255)
                                                                                          tGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGCGGGAGTGGCATCTTATCCCGCACCGCTGTACCTCTCTGAAATTGCGCCGCAAACAATTCGTGGCACTATGATCTCGCTGTATCAGTTGATGATCACTATCGGGATCCTCGGCGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGACTGGCGTTGGATGCTGGGTGTGATTATCATCCCGGCAATTTtgctgc                                                                                                   >  1:194411/1‑251 (MQ=255)
                                                                                               cAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCTCACCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAATTCGTGGCAGTATGATCGCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGAtt                                                                                              <  1:353/251‑1 (MQ=255)
                                                                                                aaaCGTTGAAGGACTTATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGGGTGGCCTCTTATACCTCCCCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAATGCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGAGCCTCTGTGCTTATCTTTCGTATACCGCCTTCAGCTTCACCTGTGTATTGCGCTGGATGCTAGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTg                                                                                             <  1:160331/251‑1 (MQ=255)
                                                                                                                                               tgggtgTGTCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCTAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCt                                                                                                                                                                            <  1:143089/125‑1 (MQ=255)
                                                                                                                                                              ataCCGCACCGCTGTACCTCTCTGAAATTGCGCCGAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGc                                <  1:182691/250‑1 (MQ=255)
                                                                                                                                                                                   ctGAAATTGCGCCGAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCCCTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGtg                      >  1:190773/1‑239 (MQ=255)
                                                                                                                                                                                           gcgcCGAAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTTGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGacac  <  1:11465/251‑1 (MQ=255)
                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
CAGCGGCTGGCTCTCCTTTAAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGGAAAAAATTCGTGGCAGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCTGATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCGGCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCCAAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACAC  >  NC_000913/3088496‑3088933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑