Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,010,929	G→A	G301D (GGC→GAC)	hyuA →	phenylhydantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,010,929	0	G	A	100.0%	17.1 / NA	7	G301D (GGC→GAC)	hyuA	phenylhydantoinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/5);  total (2/5)

CCATCGCCCGCATGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTGTCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAACCTGCCCACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGGCATCAGCGATGGTGCGATTGACGTGGTGGCAACCGATCACTGCACCTTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCGCATGCAGTTACTGTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAACGCTTTGTTG  >  NC_000913/3010683‑3011117                                                                                                                                                                                                                                                       |                                                                                                                                                                                             ccATCGCCCGCAGGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTGTCTAGCGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGGTGAAACCTGCCCACAATATCTCCTGTGGGACGAACGCATTTACGATTCAGAAGATGGCATGAAGTGCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGACATc                                                                                                                                                                                          <  1:109741/251‑1 (MQ=255)                  aaCCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTGTCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCACGTGCGAATCACAAGCCAGACTGGGTTGACACCTGCCCACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGACATc                                                                                                                                                                                          >  1:144551/1‑234 (MQ=255)                                                                                                  tGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAACCTGCCCACAATATCTCCTGTTGGACTGACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGGACGCGAGCAGGACAATCTGTGGTGTTACATCAGCGATGGTGCTATTGACGTGGTTGCAGCCGATCACTGCACCTTCTCGATGGCTCAACGTCTGCAAATGTCGAAAGGCGATTTCAGTCGCTGCCCa                                                                                          <  1:65297/250‑1 (MQ=255)                                                                                                         gCGAATCCCCAGCCAGTCTGCGTTGAAACCTGCCCACAATATCTCCTGTTGGTCGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTTGTGTGACATCAGCGATGGTGCGATTGACGTGGTGGCAACCGTTCACTGCACCTTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCt                                                                                   <  1:94392/250‑1 (MQ=255)                                                                                                                            gggTTGAAACCTGCCCACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGTTGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGACATCAGTGATGGTGCGATTGACTTGGTGGCAACCGATCACTGCACCGTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGGCGCTGCCCAAATGGCTTACCCGGTGTGGAAAAccn                                                                <  1:43092/250‑2 (MQ=255)                                                                                                                                         cccACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGACATCAGCGATGGTGCGATTGACgtggtn                                                                                                                                                                  <  1:44223/139‑2 (MQ=255)                                                                                                                                                                                         cATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGACATCCGCGATGGTGCGATTGACGTGGTGGCAACCGATCACTGCACCTTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCGCATGCAGTTACTGTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAAAGCTttgttg  >  1:143665/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                             CCATCGCCCGCATGATTAACCTGGCACAAATTGCCGGTAACGCCCCGCTCTATATCGTGCACCTGTCTAACGGCTTAGGTCTGGATTATCTGCGTCTTGCCCGTGCGAATCACCAGCCAGTCTGGGTTGAAACCTGCCCACAATATCTCCTGTTGGACGAACGCAGTTACGATACAGAAGATGGCATGAAGTTCATTCTTAGCCCACCGCTGCGTAACGTACGCGAGCAGGACAAACTGTGGTGTGGCATCAGCGATGGTGCGATTGACGTGGTGGCAACCGATCACTGCACCTTCTCGATGGCTCAACGCCTGCAAATTTCTAAAGGCGATTTCAGTCGCTGCCCAAATGGCTTACCCGGTGTGGAAAACCGCATGCAGTTACTGTTTTCCAGTGGCGTGATGACGGGACGTATAACACCGGAACGCTTTGTTG  >  NC_000913/3010683‑3011117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑