Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,994,705 T→C intergenic (+849/+1667) ygeI → / ← insD4 protein YgeI/IS2 insertion element protein InsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,994,7050TC94.1% 46.5 / ‑3.3 17intergenic (+849/+1667)ygeI/insD4protein YgeI/IS2 insertion element protein InsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (7/9);  minor base A (0/1);  total (7/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

CAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACTATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAGCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCC  >  NC_000913/2994470‑2994898
                                                                                                                                                                                                                                           |                                                                                                                                                                                                 
cAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGCCTGTTTTCCTGCTTAACTGCAAGAAATTCGCGATTTCTTTGTTTGTTTTACCATCAGCTCTGAGTTGTAATATTTCTGACTCACGATTTGTACGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCACACTAAgtgt                                                                                                                                                                                     >  1:32625/1‑250 (MQ=255)
             ntAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTCATTTCTTCATGCTATTGAGCCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAACTTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATc                                                                                                                                                                       >  1:27126/2‑251 (MQ=255)
                        ttGATCAACTCGATACCACTGTGAACATCTAATTTATTCATGATATTGAGCCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCCTATGTACACCATCAAAATAAGTGTAACCATTACTAATCGaa                                                                                                                                                                    >  1:194852/1‑243 (MQ=255)
                          gATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCAc                                                                                                                                                          <  1:1526/251‑1 (MQ=255)
                                                                                aGTTTCGACTGTTTTCCTGCTTAACTTCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGATGATTATCAGAGGAATTCCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCAGTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCtt                                                                                                    <  1:223479/251‑1 (MQ=255)
                                                                                     cGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAgn                                                                                               <  1:45237/251‑2 (MQ=255)
                                                                                                cTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAGCAATTTTAtt                                                                                      <  1:113378/249‑1 (MQ=255)
                                                                                                                      cGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTAACAATCCATATGTACACCATCAAAATAAGTGTANCCANNNNNaannnnnnnnnn                                                                                                                                                               >  2:136683/1‑144 (MQ=255)
                                                                                                                                    gtttTACCATCAGCTATGAGTTGTAANATTTCTNNCTNACGATTTGTAAGTTGATTANCANNNGNNNANCTGGANGANNTTTTTTCACNATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGNACGATTTAGNNTCGCCGGGTGACTGGTTTTCCNTACNNANNCNTTCNc                                                                                                            >  2:50351/1‑191 (MQ=255)
                                                                                                                                                               atTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAATCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAAc                     <  1:190746/251‑1 (MQ=255)
                                                                                                                                                                 ttCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAAACGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCGGCTTCAAGCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAAcc                    <  1:222403/250‑1 (MQ=255)
                                                                                                                                                                      aCTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCAGCAAAATAAGTGTAACCATTACGAATCGAATCAATATCACGATTTATTTTCGCCGCGTGTCTTGTTTGCCATACGCATCCTTTCGCACCTGCTTCAAGCAATTTGATTTAATCAATATACGGCGTCTGTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCAtttt              <  1:214896/251‑1 (MQ=255)
                                                                                                                                                                          aCGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGCTATTTTTTCACAATCCATCTGTACACCATCACAATAAGCGTAACCATTACTAATAGAATCACTAGCACGCTTTCGTTTCGCCGGGTGCCTGGt                                                                                                                                 >  1:105588/1‑132 (MQ=255)
                                                                                                                                                                                 gTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACAATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTGTCGCACCTGCTTCAAGCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTGCGGc   <  1:94731/251‑1 (MQ=255)
                                                                                                                                                                                   aaGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAGCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGcc  <  1:50351/250‑1 (MQ=255)
                                                                                                                                                                                                                      tttCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAgn                                                                                               <  1:132639/122‑2 (MQ=255)
                                                                                                                                                                                                                      tttCACAATCCATATGTACACCATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAGc                                                                                               >  2:132639/1‑122 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                 
CAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTTTGTTTTACCATCAGCTATGAGTTGTAATATTTCTGACTCACGATTTGTAAGTTGATTATCAGAGGAATACCTGGAAGATATTTTTTCACAATCCATATGTACACTATCAAAATAAGTGTAACCATTACTAATCGAATCAATAGCACGATTTAGTTTCGCCGGGTGACTGGTTTTCCATACGCATCCTTTCGCACCTGCTTCAAGCAATTTTATTGAATCAATATACGGCATCTTTGCTACAAATGTAATAATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCC  >  NC_000913/2994470‑2994898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑