Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,929	G→A	S28F (TCC→TTC)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,929	0	G	A	100.0%	22.4 / NA	9	S28F (TCC→TTC)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

AATAGTCGCCGAAGCTGAAGTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGAC  >  NC_000913/2821705‑2822114                                                                                                                                                                                                                                 |                                                                                                                                                                                          aaTAGTCGCCGAATCTGAAGTTGCGCAGCATTTCGGAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGGTGTGGTTACCACCCGCACGCACGCATCGTTGGGAAGTTGGAGCGCGGGGGTAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAGCTGGTTCCTCCCGGCGGTGGTAATCAACAAAGTTTGGTCGGTAGGGGGTTCCAGGAAGCTGCTGGCAACTACCTGATGTccc                                                                                                                                                                 <  1:95407/251‑1 (MQ=255)                                                           gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATa                                                                                                        >  1:10539/1‑250 (MQ=255)                                                                                                   cgcacgctGCGTTGGGAAGTGTTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGATAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGAGGTCCCGTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTc                                                               <  1:84326/251‑1 (MQ=255)                                                                                                                    aGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCaan                                                                                       <  1:21665/210‑2 (MQ=255)                                                                                                                                            cTTGTCGAGCCCAAGGAACACCTCCTTGAACTGGTTCATAACGGCGTTGGTAAACAACAAAGTTGTGTAGTTATGGGGTAACAGGAAACTGCTGGCAACTACCTGCTGTCCCTTACTATGGAACCAGCCGAGAAACGCCTGACGGATATCAGCGGTGCTCTTGCTCATAATAATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTggg                       >  1:139805/1‑250 (MQ=255)                                                                                                                                               gTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAat                    >  1:83987/1‑250 (MQ=255)                                                                                                                                                                naTCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGAc  >  1:40040/2‑251 (MQ=255)                                                                                                                                                                             gTTCATCCCGGCGTTGGTAAACAACCAAGTTGGGTCGTTATGGGGTACCAGGAAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCATGACGGATCTCAGCGGTGCTCTTGCTCATAATTCTCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCggg                                          >  1:113157/1‑198 (MQ=255)                                                                                                                                                                                                  caacaaAGTTGGGTCGTTATGGGGGACCAGGAAGCTGCTGGCAACTACCTGATGTCCATTACTATGGAAAAAGTCGAGAAACGCCTGACGGAACTCAGCGGTGCTCTTg                                                                                                              >  1:200570/1‑109 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                          AATAGTCGCCGAAGCTGAAGTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACACATCCTTGAACTGGTTCATCCCGGCGTTGGTAAACAACAAAGTTGGGTCGTTATGGGGTACCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGAC  >  NC_000913/2821705‑2822114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑