Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	740,179	C→T	Q225* (CAA→TAA)	phr →	deoxyribodipyrimidine photolyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	740,179	0	C	T	87.5%	12.6 / ‑3.2	8	Q225* (CAA→TAA)	phr	deoxyribodipyrimidine photolyase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (2/5);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

GCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATC  >  NC_000913/739975‑740371                                                                                                                                                                                                             |                                                                                                                                                                                                 gCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCCCCAGCCGTTTGTCgn                                                                                                                                                     <  1:54196/250‑2 (MQ=255)      gCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGcc                                                                                                                                               <  1:86172/251‑1 (MQ=255)                  gCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAAcgggcg                                                                                                                                   <  1:34949/251‑1 (MQ=255)                                  naGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGCAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTAtcgcctcgcc                                                                                                                    >  1:137735/2‑250 (MQ=255)                                                                     aTCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAgn                                                                                <  1:175460/251‑2 (MQ=255)                                                                                          catTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGt                                                            >  1:187092/1‑250 (MQ=255)                                                                                                                                ngcATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGgccacagtctttg                     >  1:23579/2‑240 (MQ=255)                                                                                                                                                   aaaaGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGTAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTTGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATc  <  1:16256/251‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                 GCCTGGCTGAAACGGCTGCGGGAAGGGATGCCGGAGTGCGTCGCTGCGCCAAAAGTTCGTAGTAGCGGATCGATAGAGCCCTCGCCATCCATTACGCTGAATTATCCTCGTCAGTCTTTCGATACTGCGCATTTTCCGGTGGAAGAAAAAGCGGCGATTGCGCAATTACGCCAGTTTTGCCAGAACGGTGCCGGAGAATATGAGCAACAACGAGATTTTCCGGCAGTGGAAGGCACCAGCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATC  >  NC_000913/739975‑740371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑