Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,109,071	T→C	I172V (ATA→GTA)	rfbX ←	polyisoprenol‑linked O16‑antigen repeat unit flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,109,071	0	T	C	100.0%	18.7 / NA	7	I172V (ATA→GTA)	rfbX	polyisoprenol‑linked O16‑antigen repeat unit flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/2);  total (5/2)

GTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAA  >  NC_000913/2108937‑2109315                                                                                                                                       |                                                                                                                                                                                                                                                     gTCTGCTAACGATCGACGCACATTATNTAATGANNGCNTAACTTTACCAATCCAACCNTCNNNANNNANGATAGNTANNGCCATAATTNCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTACATCTGACNTTGTGTTCANNAAAATAAATGTTAGAGGGATANTAGNNANNCNGGANaan                                                                                                                                                                                            >  2:48895/1‑192 (MQ=255) gTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTACATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAg                                                                                                                                     <  1:48895/248‑1 (MQ=255)              ngACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTACATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCaa                                                                                                                                                                                                      >  1:24122/2‑170 (MQ=255)                                          cTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTACATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCatat                                                                                          >  1:94117/1‑250 (MQ=255)                                                                   aacGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCTCTTGACTGAATAAAACCGGCAATTGCTACATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATGTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAg                                                                <  1:139427/251‑1 (MQ=255)                                                                                                         cACTTGACTGAATAAAACCGGCAATTGCTACATCTGACTTTGTGTTCACAAAAATCAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTCCTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGCCTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTCTATTCAGGCACAAGCAAGGTCATCGACGTTAAGCAAATCAATGt                           >  1:92556/1‑250 (MQ=255)                                                                                                                                  tGCTACATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGaa  >  1:6752/1‑250 (MQ=255)                                                                                                                                       |                                                                                                                                                                                                                                                     GTCTGCTAAAGATCGACGCACATTATGTAATGATAGCGTAACTTTACCAATCCAACCTTCATGAACAACGATAGCTAGTGCAATAATTCCAGCAACCAGATTTGCACTTGACTGAATAAAACCGGCAATTGCTATATCTGACTTTGTGTTCACAAAAATAAATGTTAGAGGGATAATAGCCAAGCGGGATAAAATACTACTTAAAGTCAGCCATTTCATTTTTTCTTTTCCCTGAAACAGCCAGATAGGGTAGATTAAATTCCCGACTAATGCAGGAACAAACGACCATATAATTACGGCATGCTTGTTATATTCAGGAACAAGCAAGGTCATCGACGTTAAGAAAATCAATGTAATGACGATAAGAACTATTTTTGAA  >  NC_000913/2108937‑2109315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑