Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,068,562	G→A	intergenic (‑1277/‑73)	insH6 ← / → yoeA	IS5 family transposase and trans‑activator/TonB‑dependent receptor plug domain‑containing protein YoeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,068,562	0	G	A	100.0%	26.4 / NA	10	intergenic (‑1277/‑73)	insH6/yoeA	IS5 family transposase and trans‑activator/TonB‑dependent receptor plug domain‑containing protein YoeA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

GTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAGATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTGGGATAGTTTCTACTGCAACCGCAACTTCTTCAGAAACAAAAATCAGCAACGAAGAGACGCTCGTCGTGACCACGAATCGTTCGGCAAG  >  NC_000913/2068416‑2068783                                                                                                                                                   |                                                                                                                                                                                                                              gTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCAtt                                                                                                                                                                                             >  1:116044/1‑181 (MQ=255)                           ccGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAACACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCAttt                                                                                             >  1:141547/1‑251 (MQ=255)                                 gACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGA‑GATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTggn                                                                                        <  1:135387/249‑2 (MQ=255)                                        gTCAGAGGCAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTCCAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTATTCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTGGGATAGTTTc                                                                                >  1:215429/1‑251 (MQ=255)                                            gagGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACATTTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTANCATNNNNNTann                                                                                                                                                                                     >  2:127932/1‑144 (MQ=255)                                            gagGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTGGGATAGtttn                                                                                <  1:127932/247‑2 (MQ=255)                                                                        aaaTATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCAttt                                                                                                                                                                                >  1:9157/1‑123 (MQ=255)                                                                                                anNGTCAAANNTGTTTGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCAttt                                                                                                                                                                                <  2:9157/99‑1 (MQ=255)                                                                                                            ntttGATATTTACAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTGGGATCGTTTCTACTGCAACCGCAACTTCTTCAGAAACAAAAATCAGCAACGAAGAGACGCTCGTCGTGACCACGAATc            >  1:39331/2‑251 (MQ=255)                                                                                                                       aCAATTTATGAAGATGACAATAATTATAAATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATGATTCATTTCTGGGATAGTTTCTACTGCAACCGCAACTTCTTCAGAAACAAAAATCAGCAACGAAGAGACGCTCGTCGTTACCACGAATCGTTCGgcaag  <  1:67591/250‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                                                                                              GTGCGGTCCGTGGATACAACCAGAGACCGACTGACACGAGTCAGAGGAAACGACGGATATGTTCAGTCGTAAAATATCTATCAAAAAACATGATTAAGGTCAAAAATGTTTGATATTTACAATTTATGAAGATGACAATAATTATAGATATATGAGAACATAAATGAAAATAATTATCATTACAGTAATCATTTGTACTTTGTATTAATGAGGGATGAAATGTTATATAATATACCTTGTCGAATTTATATCCTTTCCACTCTGTCATTATGCATTTCTGGGATAGTTTCTACTGCAACCGCAACTTCTTCAGAAACAAAAATCAGCAACGAAGAGACGCTCGTCGTGACCACGAATCGTTCGGCAAG  >  NC_000913/2068416‑2068783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑