Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,633	G→A	W580* (TGG→TGA)	cstA →	pyruvate transporter CstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,633	0	G	A	100.0%	18.2 / NA	8	W580* (TGG→TGA)	cstA	pyruvate transporter CstA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

TCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTC  >  NC_000913/631488‑631813                                                                                                                                                  |                                                                                                                                                                                     cncgcnANNANNGCGNGGTCGATCCGCTGGGCGGCATNNACACTCTGGNGCCGCTGTTTGGTATTGCCGACCAGATGCTGGCAGGGATGGCGCTGATGCTCTNTGCCGTGGTNNTGNTCAAGNTNNNANNNCANCGTTACGCCTGAGTGGCGCNGGNNCCAACGNCCTGGCTGCTGATTTGTACCCTGAcc                                                                                                                                         <  2:45856/187‑1 (MQ=255)               gTGGTCGATCCGCTGGGCGGCATTCCCACTCTGTGGCCCCCGTTTGGTATTGCCAACCAGTCGCTGGCCGGGATGGAGCTGATGATCTGAGCCGTGGTGTTGTTCAAGATGAAACGTCCACGTTCCGCCTGAGTGGCGCTg                                                                                                                                                                             >  1:210784/1‑141 (MQ=255)                                nggcATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAg                                                                                                                             >  1:127943/2‑172 (MQ=255)                                     ttAACACTCTGTGGCCGCGGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCGGTGCCGTGGTGTTGTTCAAGATGCAACGTCAACGTTACGCCTGAGAGGCGCTGGTACCAACGGCCTGNCTGNNNNntt                                                                                                                                                    >  2:137408/1‑144 (MQ=255)                                                  nnnnnnnnnnggNNNNNCCANCCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAg                                                                                                                             <  2:127943/144‑1 (MQ=255)                                                                 tGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAAGAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGgcg              <  1:161184/250‑1 (MQ=255)                                                                             gCTGGCAGGGATGTCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTGCCTGGCCATTGCTAATAAGTTCCAGGCAATTATCTACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGttn  <  1:26844/250‑2 (MQ=255)                                                                                                                                    cAACGTTACGCCTGAGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGGTGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATgctctgcgc                                                      >  1:71900/1‑135 (MQ=255)                                                                                                                                                  |                                                                                                                                                                                     TCCTCCATCAGGGCGTGGTCGATCCGCTGGGCGGCATTAACACTCTGTGGCCGCTGTTTGGTATTGCCAACCAGATGCTGGCAGGGATGGCGCTGATGCTCTGTGCCGTGGTGTTGTTCAAGATGAAACGTCAACGTTACGCCTGGGTGGCGCTGGTACCAACGGCCTGGCTGCTGATTTGTACCCTGACCGCAGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTC  >  NC_000913/631488‑631813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑