Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,303,073	Δ12 bp	coding (175‑186/921 nt)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1303073	1303084	12	7 [0]	[0] 7	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1303072	0 (0.000)	7 (0.740)	6/312	0.3	100%	coding (174/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
	?	NC_000913	1303085 =	0 (0.000)					coding (187/921 nt)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

CGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAA  >  NC_000913/1302864‑1303262                                                                                                                                                                                                                 |                                                                                                                                                                                               ngcATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggt                                                                                                                                                      >  1:25214‑M1/2‑209 (MQ=255)                            gTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGTCGCTATTTATTCTTATTACTATTTCGTTCTTTAGGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtctttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaag                                                                                                                                   <  1:13798‑M1/243‑62 (MQ=255)                                              ttATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCTCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcactgtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgc                                                                                                          <  1:35683‑M1/250‑87 (MQ=255)                                                                                                                          cgcgCCGGGAAGCCCTTTTACCGGTGAACGTCATTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatacagttttcccgtttctgccaaactgggagcctcagcatttttccttgcggtaatactgggtgttagggctgg                               <  1:145059‑M1/249‑162 (MQ=255)                                                                                                                                 ggAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggtgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgcttgcgttattgc                      <  1:62245‑M1/251‑171 (MQ=255)                                                                                                                                                      cGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGtatttcagctacctgaaacaactggcgcacggtgatttcggtccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttttcccgtttctgccaaactgggagccgcagcatttttccttgcggtaatactgggtgttagtgctggcgttattgccgcattaaaacaaaacacca  >  1:209287‑M1/1‑60 (MQ=255)                                                                                                                                                                          gttggCCAATATCGAAGCGAAATATCATCTTAATGAGCCGtatttcagctacctgagacaactggcgcacggtgatgtcgctccatcgtttaaatataaagattattcggtcaatgacttggttgcatccagttt                                                                                                 <  1:106932‑M1/133‑96 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                               CGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTCGCGCCGGGAAGCCCTTTTACCGGCGAACGTACTTTACCGCCAGAAGTGATGGCCAATATCGAAGCGAAATATCATCTTAATGATCCGATCATGACACAGTATTTCAGCTACCTGAAACAACTGGCGCACGGTGATTTCGGTCCATCGTTTAAATATAAAGATTATTCGGTCAATGACTTGGTTGCATCCAGTTTTCCCGTTTCTGCCAAACTGGGAGCCGCAGCATTTTTCCTTGCGGTAATACTGGGTGTTAGTGCTGGCGTTATTGCCGCATTAA  >  NC_000913/1302864‑1303262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.