Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.83.3% 25.1 / 3.6 12intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (8/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 9.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01
*NC_0009132,173,3640C.83.3% 25.7 / 3.0 12intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (8/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 9.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

CACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGC  >  NC_000913/2173226‑2173583
                                                                                                                                         ||                                                                                                                                                                                                                           
naCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAAATTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACAATCCatg                                                                                                           >  1:24590/2‑251 (MQ=255)
                       gCCACGAAGAAGCCGATGGTGGCAAGCTCGCCAAACGGCAGCACCTGATTGCCCAGGACACAAACAGCAATTAACATGGTGAGTGGGATATAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCCATCAGGAACTCCCGACCGCCGAAATTCGCCTGTAAACGAc                                                                                                                                                 >  1:204028/1‑190 (MQ=255)
                                   ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACCAACAGCAATTAAACTGGTGAGTGGGATCAAAATCAGGCTTGCCGCTACCACA‑‑GCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAc                                                                        >  1:161849/1‑251 (MQ=255)
                                         gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAATCAGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAGGGGCGTTAAACCATCCATGAGGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgc                                                                  <  1:121094/251‑1 (MQ=255)
                                                    gCCAAACGGCAGCACCTGATTACCCGGCACACACACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                       >  1:112113/1‑219 (MQ=255)
                                                           ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCg                                                                                                                                                                                                    >  1:91051/1‑103 (MQ=255)
                                                                                 cncaNACAGCNATTANAATGGTGAGTGGGATNAAAAGCAGGCTTGCCGATANNANC‑‑GCCGNATGTc                                                                                                                                                                                                                   <  2:91505/66‑1 (MQ=255)
                                                                                   caAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACNAGCNNNNNTann                                                                                                                                 >  2:128630/1‑144 (MQ=255)
                                                                                               nanaTNGTGAGNGGGANAAAAATCAGGCTTGCNGATACCACA‑‑GCCGTATGTCNNANCAGCANCGCCg                                                                                                                                                                                                    <  2:91051/66‑1 (MQ=255)
                                                                                                     gTGAGAGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACTCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGACTGTAAACGACTACGAGCCTGCTAAGCGATGAGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAt      >  1:127198/1‑251 (MQ=255)
                                                                                                         gTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGc  <  1:152594/251‑1 (MQ=255)
                                                                                                             gATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAANCATNNnnnatnnnnnnnnnn                                                                                               >  2:133573/1‑144 (MQ=255)
                                                                                                                                         ||                                                                                                                                                                                                                           
CACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGC  >  NC_000913/2173226‑2173583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑