Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,676	0	A	G	53.3%	‑5.9 / 10.1	15	D70D (GAT→GAC)	tfaQ	putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base G (4/4);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTC  >  NC_000913/1634473‑1634819                                                                                                                                                                                                             |                                                                                                                                                tGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATACGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGT‑TTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGTCAc                                                                                                  >  1:104469/1‑251 (MQ=11)                   ntttCTTCCGCCTCACGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCGCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGGCATAGACGGT‑TTTACCCCGATGGTCTTCAACgag                                                                                                                                      >  1:68626/2‑197 (MQ=11)                            gCCTCCCGTATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTTTGCCGGTCCCCTTCTGATATTCCCCTCCCGGCGATTACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCAGAGCCGGT‑TTTACTCCGTTGGTCGTCAGCTAGATGCCACGATGCCTCATCACTGTTGAAAACAGGCACAAAGCCAGCCGGAATATCTGGCGGTGc                                                                       <  1:218550/251‑1 (MQ=11)                                                                 ccTTCACCCAGGCGGTGCCTTTCCGCTTCTGATATTCCCCGCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGGCATAGAC‑GTGTTTACCCCGATGGGCTTCAACGAGATGCCGCGAGGCCTCATCACTGTGGAAAACAGCTACAGAGCCAGCCGTAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAgg                                   <  1:164739/250‑1 (MQ=2)                                                                     cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGATCACATTTTTCGGTAACGGACCGAGTTCAGAAATACATAACGCGTCGCCGGAAGACACGTCATCAACCGA‑TTTCACCCGTTGATCTTCACCGAGATGACACGATGCCTCATCACTGTTGAAAACAGACACGAAGCCAGCCGGAATATCTGGCGGGGCAATATCGGTACTGTTTGCTGGCCGACCTGTATGAGGCGGa                               >  1:182271/1‑250 (MQ=11)                                                                               gtgCCGTTCCACGTCTGAAACTCCCCTTCCGGCGATAACCATGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACTTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATAGCTGGCGGTGCAATATCGGTTCTGTTTGCTTGCAGGCCTGTATGAGGCGGAATATATGCATc                    <  1:105402/251‑1 (MQ=11)                                                                                    gTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTCAAGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGT‑TTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAagccag                                                                                          >  1:34643/1‑176 (MQ=11)                                                                                               gAAACTCCCCGTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACGCATCACTGGTGAAAACATCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTgg                                                  <  1:240935/205‑1 (MQ=9)                                                                                                     ncccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGc                                                                            >  1:26265/2‑173 (MQ=17)                                                                                                              gCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCAAGTCATAAACCGT‑TTTACCCCGATGATCTTCAAAGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc                                                            >  1:185360/1‑180 (MQ=17)                                                                                                                    aCCAGGTAAAATGTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGT‑GTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTTTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAc                                             <  1:42662/189‑1 (MQ=11)                                                                                                                                      gTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGT‑TTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTc  <  1:59382/214‑1 (MQ=11)                                                                                                                                               cGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCNNCACTGTNNa                                                                                                            >  2:14298/1‑99 (MQ=21)                                                                                                                                                  gTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGCTGCCACGATAACTCTTCACTgatg                                                                                                             >  1:112337/1‑92 (MQ=11)                                                                                                                                                                    cgTCGCCGGAAGCCACGTCATAGACGGT‑TTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAg                                    >  1:98869/1‑150 (MQ=11)                                                                                                                                                                                                             |                                                                                                                                                TGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGT‑TTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTC  >  NC_000913/1634473‑1634819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑