Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	579,717	0	A	C	52.9%	‑5.8 / 17.7	17	intergenic (‑123/‑163)	ybcV/ybcW	DUF1398 domain‑containing protein YbcV/uncharacterized protein YbcW
Reads supporting (aligned to +/- strand):  ref base A (2/6);  new base C (4/5);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACC  >  NC_000913/579523‑579951                                                                                                                                                                                                   |                                                                                                                                                                                                                                           aTAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGCATATTTCTCTAACAATGCCAACCTGAGCCATTGGTCAAAACTTCCATGTGATACGACGGCGCGTAGTTTGCCTTATCGTTTTTCTCGCTTCAATCTGGTATGACCTCTTTGTGTTTTGTTGATGATTTATGTCAACTATTAGGCATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGAGCGGTCCTGCTGGc                                                                                                                                                                                                   >  1:235664/1‑236 (MQ=255)                              gCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCCTGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTCTGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAg                                                                                                                                                                                          >  1:119024/1‑216 (MQ=255)                                       cGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCaaat                                                                                                                                              >  1:62614/1‑250 (MQ=255)                                              ttCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTGCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCAt                                                                                                                                        <  1:147127/250‑1 (MQ=255)                                                     aaaaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATCCGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTGTTGTTGATGATTTATGTCAAATATTAGGAAGGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGGGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACg                                                                                                                                                       <  1:122982/228‑1 (MQ=255)                                                                atctgCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCTCTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACATATGTATGTAAGTCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAgn                                                                                                                       <  1:50665/245‑2 (MQ=255)                                                                                                              tGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGGTTTGTTGATGATTTACGTTAAATATTCGGAATGTTTTCGCTTCACAGTATTGGTTGCGTAACACATTGCGGTCCTGCTGGCATTCTCGAGGGAACTACAACCGAAAGCTGTATGTCCGAAAAACGAGCCCACATCCTCCTACAGAAAGCTTTGAAGAAATCTTTTAACCGCTAGATGAAGAGCAAGCCCAAGGAGCGACAAAAt                                                                        >  1:236165/1‑250 (MQ=255)                                                                                                                 aTTATCGTTTTTATCGTTTCAATCTGGACTGACCTCCTTGGGTTTTGTTAGTGATTTATGTAAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTTACAAAGAGCGGTCCTGCTGGCCTTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATATTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAACGCATGGAGAGACAAAATGAAt                                                                    >  1:99972/1‑251 (MQ=255)                                                                                                                                gCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATTTTTTCAATTAATAGTATTGGTTGCGGAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATctgct                                                     <  1:236990/251‑1 (MQ=255)                                                                                                                                         tGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATGGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGTTAGATGAAGAGCAAGCGCATGGAGc                                                                                <  1:208456/215‑1 (MQ=255)                                                                                                                                          ggTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATGGGTAGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGGATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCGAGATGAATAGCAAGCGCATGAAGCGACAAAATGAATAAAGAACAATCTGCTgatgatn                                              <  1:55345/248‑2 (MQ=255)                                                                                                                                                    ccttGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATc                                  <  1:18547/248‑1 (MQ=255)                                                                                                                                                    ccttGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGANc                                  <  1:198421/248‑1 (MQ=255)                                                                                                                                                              tggtgatgaTTTATGTCAAATATTAGGAATGTTTTCACCTAATAGTATTGGGTGCGTAACAAAGTGAGGTTCTGCTGGCATTCTGGAGGGAAATACAAACGACAGATGTCTGTAAGGCCACAGTGCTCAAATCTTCCTAc                                                                                                                                      >  1:129154/4‑140 (MQ=255)                                                                                                                                                                        ttATGTCAAATATTAGGAATGTTTTCACTTAATAGGATTGGTTGCGTAACAAAGGGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCAGACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATg              <  1:194234/250‑1 (MQ=255)                                                                                                                                                                           tGTCAAATATTATTAATGTTGGCAATTAATAGTATTGGTTGCGTAACAAATTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGcc                                                                                                                                                           <  1:170852/106‑1 (MQ=255)                                                                                                                                                                                   atTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCAcc  <  1:122800/251‑1 (MQ=255)                                                                                                                                                                                      nggNATGNTNNCNNTTANTAGTATTGGTTGCGTAACAAANNGCGGTCCTGNTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCNAATCTTCATNNAGNAAGAGNTNNNGNNNTANTTTAACCGCTAGATGAAGANCANNCGCATGNAGCGACAAAATGAATAAAGAACAATc                                                         <  2:62614/192‑1 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACC  >  NC_000913/579523‑579951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑