Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,5400TC50.0% ‑1.8 / 11.8 14N19D (AAC→GAC) ybcVDUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base T (4/3);  new base C (1/6);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGC  >  NC_000913/579300‑579758
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          
cTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTAGAGAATTTAACATTTACAACCTTTTTAAGACCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCtttt                                                                                                                                                                                                                  >  1:226712/1‑251 (MQ=255)
        gCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATGAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTg                                                                                                                                                                                                          <  1:15396/251‑1 (MQ=255)
                    cTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCgn                                                                                                                                                                                                               <  1:77177/234‑2 (MQ=255)
                              nnnntntgNNTTCTNTTTATTTCAACCTATCATAGGACTNTTCTATAAGATGCGTATTGCTTGAGAATTTANCNTTTACAACCTTGTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTNACACAATGTGNATANNATCTNTNGNTAGATAGTAAATATAATGTGAGACATTGTNACGGTTTAGTTCAGAATAAAACANNTCNNNGTNNAAATc                                                                                                                                                                                                                      <  2:187356/213‑1 (MQ=255)
                                                             aTAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACTACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACGCGATGTGAATATTATCTGTTGCTATATTGTAAAGATGATGGGAGAGGGTGTGACGGGTTAGTTCTGAAGAAAACAAATCACAGTCTAAAGCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAAc                                                                                                                                                                               <  1:122317/225‑1 (MQ=255)
                                                                       tCTATAAGATGAGCATTTCTTAAGAAGTTAAAAGTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTAACGATTTCTAACACAATGTGAATAATATCTGCGGCTCGATAGTAAATATAAGGTGAGACATTGTGACGTTTTAGTACAGAATAAAACAATTCCCAGTTTAAATCTTTTAGCACTTGATCGAATATTTCTTTAAAAATGGCAAACTGAGACATTGTTAAAACCTTCAATGTGATACGAgg                                                                                                                                           >  1:197632/1‑251 (MQ=255)
                                                                        cTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGACCTTTTATTAACACGGTGTTATCGTTTTCTAACCCAATGTGAATATTATCTGTGGATAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCTGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGa                                                                                                                                                                           >  1:252186/1‑218 (MQ=255)
                                                                                                      naTTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTcaaa                                                                                                                                                                                       >  1:29761/2‑176 (MQ=255)
                                                                                                         ttACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTGAGTTCAGAATAAAACAATGCACAGTCTAAATCTTTTCGCACTTGATCTAATATTTCTTTAAAAATTGCAACCTGAGCCAGTGGTAAAACCTTCCATGTGTTGCGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTc                                                                                                          <  1:115488/250‑1 (MQ=255)
                                                                                                              aCCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCGTTAAAAATng                                                                                                                                                                                   <  1:199187/172‑1 (MQ=255)
                                                                                                                      aaGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGTCATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGAcc                                                                                            <  1:223055/251‑1 (MQ=255)
                                                                                                                               tattaACACGGTGTTATCGTTTTCTAACACGATTTGAATATTAGCTGGGGCTGGATAGTAATTAGAATGTGAGACTTTGGGACGTTTTAGTTCTGAATATAACAATTCACATTCTAAATCTTTTCGCTCTTTATCGAATATTTCTTTaaa                                                                                                                                                                                        <  1:232116/150‑1 (MQ=255)
                                                                                                                                                cGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATg                                                                  <  1:16787/251‑1 (MQ=255)
                                                                                                                                                                              tgGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTATTTCAGAATAAAACAAGTCACAGTCTAAATCTTTTCGCACTTGAGCGAATATTTCTGTAAAAATGGCAACCTGAGCCGTTGGTAAAACCTGCCATGTGATACGAGGGCGCGTAGGTTGCATTATCGGTTGTATCGTCTCAATCTGGTCTGACCTCCTTGTGTTttgtt                                                                             <  1:103113/210‑1 (MQ=255)
                                                                                                                                                                                                                               aTAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGCATATTTCTCTAACAATGCCAACCTGAGCCATTGGTCAAAACTTCCATGTGATACGACGGCGCGTAGTTTGCCTTATCGTTTTTCTCGCTTCAATCTGGTATGACCTCTTTGTGTTTTGTTGATGATTTATGTCAACTATTAGGCATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGAGCGGTCCTGCTGGc  >  1:235664/1‑236 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          
CTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGC  >  NC_000913/579300‑579758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑