Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,495,683	C→T	G176D (GGT→GAT)	ahr ←	NADPH‑dependent aldehyde reductase Ahr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,495,683	0	C	T	100.0%	13.1 / NA	7	G176D (GGT→GAT)	ahr	NADPH‑dependent aldehyde reductase Ahr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

GGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTTTATAGCGATATGCCCCAGCCCGCCAATACCAATTACCCCAACGCGGCTGGTAGCAGTGATATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTCCGCACGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGGCACCGCACCTTGCTCGCAGTTGATCTGATTACC  >  NC_000913/4495470‑4495897                                                                                                                                                                                                                      |                                                                                                                                                                                                                       ggcggccagcggAGGCTGACGTTGCTGGTTTTAAAAATGAGACCAACCGCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGTCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCGTCTCTTTCGCCGGTTTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTTTATAGCGATATGCCCCAGCCCGCCAATATCAATTCCCCCAACGCGGCTGGTAGCAGTGATATGGTg                                                                                                                                                                                   <  1:127497/240‑1 (MQ=255)       cAGTCGAGGCTGACGTTGACGGAGTTGATAATGAGATCAAACTGCCCCGCAGGTGCTTTCAGTACCTGCGGATAGAGGCTATTCAACACTTTATCGGCACCCATCGCAAGCACTTACTGCTCTTTCGCCGGATTCGAACTAAAGGCTGTAACCTCGCATCCCATTGCGTGCAGCAGTTTTATAGCGATATGCCCCAGCCCGCCAATATCAATTACCCCAACGCGGCTGGTAGCAGTGTTATGGTGCATCa                                                                                                                                                                              >  1:64763/1‑250 (MQ=255)                                                                                                     cGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTTTATAGCGATATGCCCCAGCCCGCCAATATCAATTACCCCAACGCGGCTGGTAGCAGTGATATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTc                                                                                <  1:53529/250‑1 (MQ=255)                                                                                                                                                 gtcctcttccgttcttcccATCCCAGTGCGTGAAAAAGTTTTTAAGGAATAGGCCCCACCCCGCCAATATCAATTACCCCAACGCGTCTGGTAGCAGT‑‑TATGGTGCATCAACAGTGGTTTAAAGACCGTGAGACCGCCGCACAACAGCGTCCCGGCGGTCTCGATATCAATATTTGCTGGCAGGGGAATCACCCATTGCCAGTCCGCGCGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGgc                                  <  1:24589/232‑1 (MQ=255)                                                                                                                                                    aaGGCTGTCCCCTCGCATCCCATTGCGTGCAGAAGTTTTATAGCGATATGCCCCAGCCCGCCAATATCAATTACCCCAACGCGGCTGGTAGCAGTGATATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTCCGCACGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGgcac                                <  1:137317/251‑1 (MQ=255)                                                                                                                                                                                   gAAGTTTTATAGCGATATGCCCCAGCCCGCCAATATCAATTACCCCAACGAGGCTGGTAGCAGTGCTATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTCCGCACGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGGCACCGCACCTTGCTCGCAGTTGATCTGATTAcc  >  1:163195/1‑250 (MQ=255)                                                                                                                                                                                                        ccAGCCCGCCAATATCAATTACCCCAACGCGGCTGGTAGCAGTGATATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTCCGCACGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGGCACCGCACCTTGCTCGCAGTTGATCt         >  1:4395/1‑222 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                       GGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTTTATAGCGATATGCCCCAGCCCGCCAATACCAATTACCCCAACGCGGCTGGTAGCAGTGATATGGTGCATCAACAGTGGTTTAAAGACCGTGATACCGCCGCACAACAGCGGCCCGGCGGACTCGATATCAATATTTTCTGGCAGTGGAATCACCCATTGCCAGTCCGCACGCAACTTCTCGGCAAAGCCACCGCGATTCATAATCGTCGGCACCGCACCTTGCTCGCAGTTGATCTGATTACC  >  NC_000913/4495470‑4495897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑