Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,777	G→A	R255R (CGG→CGA)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,777	0	G	A	100.0%	24.5 / NA	10	R255R (CGG→CGA)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

CAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGC  >  NC_000913/4156583‑4157018                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  cAACGCATTGCCGAAATGACAGCCGCGAAAGCAGCACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTgg                                                                                                                                                                                                                             >  1:144587/1‑217 (MQ=255)           ccGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTCCTGACGGCATGATCGTGAAAGTGCACGCGGCGCCGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTACCGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAAc                                                                                                                                                                                 >  1:76965/1‑251 (MQ=255)                    ccgccgCGAAAGCATAACAACTTATTGAGCAGGGCATTATTACTGACGGCATGATAGTGACAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGGCCGGTAGATATCGCCTCCTGGCGGCATGCGGAGCAGCTTCCAGCACTGTTGAACGGTATGCCGATGGGTACGCGAATTTTATCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATg                                                                                                                                                                        <  1:122813/251‑1 (MQ=255)                           naaaGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTcc                                                                                                                                                                                                           >  1:108597/2‑209 (MQ=255)                                          gATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGtttt                                                                                                                                                   >  1:57502/1‑250 (MQ=255)                                             tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAcn                                                                                                                                                <  1:102978/250‑2 (MQ=255)                                                                                                  ntGCNGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTcc                                                                                                                                                                                                           <  2:108597/137‑1 (MQ=255)                                                                                                                                              cGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTCGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCAATGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCa                                               >  1:125661/1‑250 (MQ=255)                                                                                                                                                                                    gCCGATGGGTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGACGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTGGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAagag         <  1:172783/250‑1 (MQ=255)                                                                                                                                                                                          gggTACGCGAATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCGTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTGCAAACAATTCAACGACTCACTGCTCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCGGGTCCAAAGCCCGTGTCACTGTAGGCGTGTTAACCGCAGAAGAGCAGgcgc  <  1:154211/251‑1 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  CAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCACTGCGCTTTGATTACCGTCTGGCGGAGCAGGATATTGTTGGCTCTGTGGCCTGGTCCAAAGCCCTGGTCACGGTAGGCGTGTTAACCGCAGAAGAGCAGGCGC  >  NC_000913/4156583‑4157018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑