Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	557,635	A→T	L36Q (CTG→CAG)	folD ←	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	557,635	0	A	T	100.0%	34.4 / NA	13	L36Q (CTG→CAG)	folD	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (7/6);  total (7/6)

TCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGG  >  NC_000913/557425‑557804                                                                                                                                                                                                                   |                                                                                                                                                                          tCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCCTCGATGGTGTTGTCGGCATTAAGAGTATCGATAAGCTCCAGCAGCTCCGCTTAGATGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGCCGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCCCCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa                                                                                                                                    >  1:125887/1‑250 (MQ=255)         cgccgGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATGTGCGGTGCAGGGTTACTACCCACCAGCGCAACTGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGan                                                                                                                           <  1:16632/251‑2 (MQ=255)           ccgGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc                                                                                                                          >  1:39115/1‑250 (MQ=255)                                    gCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg                                                                                                              <  1:145213/237‑1 (MQ=255)                                         cGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGGAGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACCTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt                                                                                                                              >  1:117142/1‑216 (MQ=255)                                                             aGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg                                                                                                              <  1:53867/212‑1 (MQ=255)                                                                                             gCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTTACAAGACTTGCGTTTGCTTGCGACATACATTTGCGATGCAGGGTTAATACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATc                                                                            >  1:163211/1‑214 (MQ=255)                                                                                                              ggTCATAAGAGCGGGAGACTAACCCGACTTCTTCACAAGCCTTGCTTTTGCTTGCGACATAAATTTGCGATGCAGTGTTACTACCCACCAGCACAACGGCCTGTCCTGTTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCACCTTCagag                                                                                                                <  1:109273/161‑1 (MQ=255)                                                                                                              ggTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTGTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTtgctgc                                                                    <  1:90166/205‑1 (MQ=255)                                                                                                                                                                       cATNAATTTGCGATGNAGGGTTACTACCCACCAGCACAACGGCCNGTCCTGGTGcc                                                                                                                                                                <  2:22166/56‑1 (MQ=255)                                                                                                                                                                       cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGACTGTCCTGGTGca                                                                                                                                                                >  1:22166/1‑55 (MQ=255)                                                                                                                                                                        ntANATGNGNNANNCAGNGTTACTACCCACCAGCACAACNNCCTGTCCTGNTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCNCACCTGCTGNNCANGCGTGNTNNNGNNNATNATCTTTGCTGCCATCAGAGNGANNATTCCANCTGGTACGTAGATCGAAGGCGATgca                      <  2:39115/192‑2 (MQ=255)                                                                                                                                                                                 cGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCCGTCAGGCTGCAATACGCGCCTgcccttt                                                                                                                                >  1:83564/1‑72 (MQ=255)                                                                                                                                                                                      cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCAGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGCAGGGGATGCGCCTATTTTGTCAGAAGCggg  >  1:142016/1‑199 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                          TCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCCAGCAGCTCCGCTTCGCTGGTGGTTTCCGGGAGGTCATAAGAGCGGGAGACGAACCCGACTTCTTCACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACCTGCTGCGCAATCGTTTTACCGTCAATAATCTTTGCTGCCATCAGAGAGAGGATTCCATCTGTTACGTAGATCGAAGGGGATGCGCCTATTTTGTCAGAAGCGGG  >  NC_000913/557425‑557804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑