Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,756,336	G→A	S59L (TCG→TTG)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,756,336	0	G	A	100.0%	10.3 / NA	6	S59L (TCG→TTG)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/1);  total (5/1)

GAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTGCGCAGCATCCAGCGCCAGATCGATGTCTCGTTTGCCCGAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGA  >  NC_000913/3756105‑3756561                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  gAGGCGAAATAGCGTAAATTGTCAATCGCCAGTGGTGCTTCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTGGTTCCAGTCGATCGTCAATCTTAAACAGAATCGCCGCACGATCCTGCGCCTAGGTTTGCTCCCATTTATCGTTCACTTTGTGCTCAGCATCCAGCGCCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCAcag                                                                                                                                                                                                                <  1:118454/251‑1 (MQ=255)                                                                ggTTTGCCGTTATACCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTGCGCAGCATCCAGCGCCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTc                                                                                                                                                                   >  1:46888/1‑233 (MQ=255)                                                                 ntttGCCGTTATACCAGGTTTCAGCTGTCTCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTGCGCAGCATCCAGCGCCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTcg                                                                                                                                                >  1:41846/2‑251 (MQ=255)                                                                                                                                                                                                      agcaTCCAGCGCCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTCGCCGCCAATAAAGTTGTCATAGCGGGCttt                                                                                                                   >  1:153529/1‑147 (MQ=255)                                                                                                                                                                                                         ntCCAGCGCCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCggg                                                                                     >  1:103819/2‑174 (MQ=255)                                                                                                                                                                                                                gcCAGATCGATGTCTCGTTTGCCCAAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGACTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGa  >  1:112785/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  GAGGCGAAATAGCGGAAATGGTCAATCGCCAGCGGTACATCCGCAGCACTGGTTTCGCGAATGGGTTTGCCGTTATCCCAGGTTTCAGCTGTCGCTAACAGCTCGAGGTTTTGTTCCATTCGATCGGCAATCTTAAACAGAATCGCCGCACGATCCTGCACCGAGGTGTGCGCCCATTTATCTTTCACTTTGTGCGCAGCATCCAGCGCCAGATCGATGTCTCGTTTGCCCGAAGACGCCACTTCGCACAGCAGCTGCCCGGTCACCGGCGTCAGATTCTGGTAATACTCGCCGTCGGCAGGGGCTACCCATTCGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGA  >  NC_000913/3756105‑3756561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑