Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,596,459	C→T	A368T (GCC→ACC)	livK ←	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,596,459	0	C	T	100.0%	30.3 / NA	12	A368T (GCC→ACC)	livK	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCACCGCCGCGTAGGTGATCCAGACATAAGGCCCGGACGGATCTTTCTTGTCT  >  NC_000913/3596271‑3596691                                                                                                                                                                                             |                                                                                                                                                                                                                                         cGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGGGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGc                                                                                                                                                                            <  1:28504/251‑1 (MQ=255)                     aTGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCGGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGt                                                                                                                                                                                                                   <  1:73700/192‑1 (MQ=255)                       gCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAACCCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCg                                                                                                                                                       <  1:46188/250‑1 (MQ=255)                                  cGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACCAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCCTTTTACGGGCGGTGGGATGCTCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACgg                                                                                                                                           >  1:121935/1‑251 (MQ=255)                                                                                    gTGACGCCGTTAAACATCTGCTGCAAGAAATACACAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGa                                                                                                                                                                                                          >  1:40547/1‑138 (MQ=255)                                                                                    gTGACGCCGGTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACANAAGGTAACCNNTCNAAACCNGNNNGNNNTTNACGGGCGGTGGGATGATCANTTNNTTGCCGNGGATGAACCGTCGGCGTGCCACTGGa                                                                                                                                                                                                          <  2:40547/138‑1 (MQ=255)                                                                                        cGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGATAACCTTTCTAAACCCGCCCGCCTTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGACCCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTCGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGc                                                                                     >  1:125481/1‑251 (MQ=255)                                                                                            gTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATcccn                                                                                                                                                                                     <  1:49353/151‑2 (MQ=255)                                                                                                     ctgctgCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGc                                                                                               <  1:46772/228‑1 (MQ=255)                                                                                                                           tGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTc                                                                                                                                                                                                                        <  1:4005/85‑1 (MQ=255)                                                                                                                           tGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGTTGCCGTGGATGAACCGTc                                                                                                                                                                                                                        >  2:4005/1‑85 (MQ=255)                                                                                                                                                                           ggTGGGATGATCACTTGGTTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACAACACCATCAAATCCCTTAAGATCGACTTTTTCATCCCCGTTCAGCGGCCCAATCACGGTGTTTGCACAGTTAGCTTTTAAATCTTTCACAAGCGCCAGCGGCTCATCGCTGCCGGTCCGCTCAAGGGCAGTCGCCAGAGATTGCACCGCCGCGTAGGTGATCCAGACATAAGGCCCGGACGGATCTTTCTTGTCt  >  1:94356/1‑251 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                                         CGCCGTGGGCGAAGTTGATCATGCCGATAATGCCGTAAACCATGGTGTAGCCGATGGCTATCAGCGCGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCATCGCTGCCGGTACGCTCAAGGGCAGTCGCCAGAGATTGCACCGCCGCGTAGGTGATCCAGACATAAGGCCCGGACGGATCTTTCTTGTCT  >  NC_000913/3596271‑3596691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑