Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,582,140 C→T G213D (GGT→GAT)  ypfG ← DUF1176 domain‑containing protein YpfG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,582,1400CT80.0% 17.5 / ‑4.7 10G213D (GGT→GAT) ypfGDUF1176 domain‑containing protein YpfG
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (6/2);  total (8/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAG  >  NC_000913/2581900‑2582346
                                                                                                                                                                                                                                                |                                                                                                                                                                                                              
cgcgATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGCTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCg                                                                                                                                                                                                      >  1:67298/1‑251 (MQ=255)
                  gTTGCGTTCATCAGTTCCAGTTCATTCGTCTCATGACCGTTGTTGAACGGCACACGCCACCGAACCCGGCGCGACGCCAGTGCCTTCTTCCGCGCCACAATCCATGCCACATCCATGGTGTTATCGGCCCCTCCCTACCAGCTAATCATCATCAGCGCTTTATCCTCAGTCAGCCCAGTGACATTCACCTCACGACGCAATGGACCAAACGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATcc                                                                                                                                                                                      >  1:86859/1‑249 (MQ=255)
                      ngTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAATCCAGCaa                                                                                                                                                                                 >  1:41756/2‑250 (MQ=255)
                                                                    cAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAacc                                                                                                                                   >  1:159526/1‑250 (MQ=255)
                                                                          cAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACcgcg                                                                                                                             >  1:61890/1‑250 (MQ=255)
                                                                                                                      tCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCAAAGCTAATCATACTCCGCGCTTTATCCTCAGTCAGCGCAGTGACATTCCCCCCACGACGCCATGGATCAAGCGAGCAGAGCAGACCATTCATCCGCCAGTTTCCATAATcc                                                                                                                                                                                      >  1:163083/1‑149 (MQ=255)
                                                                                                                                      ggTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGGACAGATCATTCATCCGCCAGATTCCATAAACCAGCAAATCTTTGCGTTCTTCGAGTGAGAGTGGCGTCCCCGTTGTGTTAACCACCGCGACCTCTGTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATc                                                                 <  1:173305/250‑1 (MQ=255)
                                                                                                                                                 ncccTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATCCACGCGGttcac                                                     >  1:104573/2‑248 (MQ=255)
                                                                                                                                                   ncTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGt                                                                                                                                                                            >  1:139747/2‑130 (MQ=255)
                                                                                                                                                                                                     gACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGATCATTCATCCGCCAGTTTCCATAAGCCAGCAAATCGTTGCGGTCTTCGAGTGAGAGGGGCGTCGGCGTGGGGTTAACCGCCGCGACCTCTTTCAGCGCGGTCGCGGGCGGTACGCTTAGCGGCGGTGCGTCCCCCTTCTTGATCCACGCGGTGTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAg  <  1:83189/250‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                              
CGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGCAAACGCAACCGAACCGGGCGCGAAGCTAGTGGCTTTTTACGCGACACAATCCATGCCAAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAG  >  NC_000913/2581900‑2582346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑