Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,219	G→A	P55P (CCG→CCA)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,219	0	G	A	100.0%	18.8 / NA	10	P55P (CCG→CCA)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

ATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAC  >  NC_000913/497991‑498458                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aTTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACACGTTGAATCGATCAGAGGCGGTAATGCGTCAGACTAAAACCGGTATACTGCTGGTAAACCTGGGTAAGCCCGATGCCCCCACACCTGAAGCGGTTAAAAGCTAACTGAAAAAATTTTTAAGCGACAGACGCGTGGTTGATCCCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTCGCCGCGTgcggcg                                                                                                                                                                                                                            >  1:153717/1‑250 (MQ=255)                                                      aaGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAgc                                                                                                                                                                      <  1:153433/251‑1 (MQ=255)                                                                    cGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAAcagc                                                                                                                                                         >  1:27552/1‑250 (MQ=255)                                                                       cAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCGCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAggn                                                                                                                                                     <  1:50348/251‑2 (MQ=255)                                                                           cTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACTTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTcgccg                                                                                                                                                                                                                                     <  1:100783/167‑1 (MQ=255)                                                                                                                     nccccACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGc                                                                                                       >  1:102370/2‑251 (MQ=255)                                                                                                                                                                            cgTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCGGCGCGGCGGGATTTTGCCACTGCTCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGTATGGAAGGTGGCTCGCCGCTGATGGTTTCCAGCCGCAAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAg                                                                                                                     <  1:28539/182‑1 (MQ=255)                                                                                                                                                                                nttGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCACTGAGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATc                                             >  1:20497/2‑250 (MQ=255)                                                                                                                                                                                                                          ntGATTTTGCCACTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGAAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAc  >  1:107135/2‑251 (MQ=255)                                                                                                                                                                                                                            gATTTTGCCACTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACTGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTTCTGCCGCTTTATCCGCAATTCTCCTGttn     <  1:33450/246‑2 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCACTGGAAAGCGCCGTAGATGAACTCCTGGCAGAGCATGTAGATCATATTGTGGTGCTGCCGCTTTATCCGCAATTCTCCTGTTCTAC  >  NC_000913/497991‑498458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑