Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,141	G→A	T54I (ACC→ATC)	thiM ←	hydroxyethylthiazole kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,141	0	G	A	100.0%	19.9 / NA	9	T54I (ACC→ATC)	thiM	hydroxyethylthiazole kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/6);  total (3/6)

GCATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATT‑GGTCATGCAGTGCACAAGAGGGGAATGTTGG‑TGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCA  >  NC_000913/2184919‑2185373                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           gCATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCAcn                                                                                                                                                                                                                <  1:50157/251‑2 (MQ=255)                                                                       cacccaccGCTACTGGATCAAGCGTCCAGGGTGTTTGAGCGCTTTTTGCTTGCTCAACGGAAGAACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGACTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATAATT‑GGTTATGTAGTGctca                                                                                                                                                  >  1:112705/1‑239 (MQ=255)                                                                               gCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAATGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATT‑GGTCATGCAGTGCACAAGAGGGGAATGTTGG‑Tg                                                                                                                                <  1:13652/251‑1 (MQ=255)                                                                                                           gagaGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGATGCGTCAGTGTGCAAACGTTCATCAACATGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTTGGCCTTG‑AGGGCACAAGAGGGGGGTGTTGGGTGAAAA‑GGTGGTACGCGTgcgccggtttg                                                                                                    >  1:154272/1‑244 (MQ=255)                                                                                                                     tgcttgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTTACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGGAGTGGCGGTAAAGTTTTTCACCACATCATT‑GGTCATGTAGTGCACAATAGGAGAATGTTGG‑TGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAAccc                                                                                           <  1:80066/250‑1 (MQ=255)                                                                                                                                        cGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCCACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCCCATCCTT‑GGGCATGCCGTGGACAAGAGGGGAATGGTGG‑GGAAAAAGGGGGAACGCGTGCGCAGAATGGGCTGGACCCAGCAAGGCGACTTGCATAgg                                                                       >  1:72797/1‑250 (MQ=255)                                                                                                                                               ccTGAGNNCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGATTTCGATAACCATCg                                                                                                                                                                                                                             <  2:482/96‑1 (MQ=255)                                                                                                                                                                                       caaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCTATTGCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATT‑GGTCATGCAGTGCACAAGAGGGGAATGTTGG‑TGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGAc                        <  1:72343/251‑1 (MQ=255)                                                                                                                                                                                                             aaCTGACTGGCCTCTTCGATTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGAGGTAAAGGTTTGCACCACATCATT‑GGTCATGCAGTGCACAAGAGGGGAATGTTGG‑TGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGACCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATTACATGGTGGTCCGTGACTTCCCTACGCTGGCATTATccn  <  1:33431/251‑2 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           GCATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATT‑GGTCATGCAGTGCACAAGAGGGGAATGTTGG‑TGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCA  >  NC_000913/2184919‑2185373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑