Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,180,087	G→A	E423K (GAA→AAA)	yegT →	putative transporter YegT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,180,087	0	G	A	100.0%	27.0 / NA	10	E423K (GAA→AAA)	yegT	putative transporter YegT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGACCGTTTTCTTCCTGGACCAAAGGAGAATAACGCGGCCTGTTATTTTAACCGCGCCGAATTCACCGACGATACCTCGATGGCGCTGTGTCTGGCGGATGCGTTACTG  >  NC_000913/2179897‑2180316                                                                                                                                                                                               |                                                                                                                                                                                                                                      gatgatGGAAAAGATGTTCGCTTAGCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGc                                                                                                                                                                           <  1:152327/251‑1 (MQ=255)    gatgGAAAATATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGGCCGGGATGTGGACGTTCGGCGCGGTTATGAGGGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAg                                                                                                                                                                                                                                    <  1:113230/191‑1 (MQ=255)                                                                                                      ccGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGctc                                                                                                                                                                                                    <  1:134145/125‑1 (MQ=255)                                                                                                                     tttttttCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAgggn                                                                                                                                                                               <  1:107471/131‑2 (MQ=255)                                                                                                                     tttttttCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAgggg                                                                                                                                                                               >  2:107471/1‑131 (MQ=255)                                                                                                                             cgcgAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTCTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGAc                                                                                                           >  1:171453/1‑191 (MQ=255)                                                                                                                                         aaCGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGACCGTTTTCTTCCTGGACCAAAGGAGAATAACGCGGCCTGTTATTTTAACCGCGCCGAATTCACCGACGATAcc                                   >  1:1534/1‑251 (MQ=255)                                                                                                                                                    ggCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGACCGTTTTCTTCCTGGACCAAAGGAGAATAACGCGGCCTGTTATTTTAACCGCGCCGAATTCACCGCCGATACCTCGATGGCGCt                        >  1:61113/1‑251 (MQ=255)                                                                                                                                                                          gATATTGCGTTGACACAAGGGAAAGTTAAATGAAAACAGAACGTATGCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGACCGTTTTCTTCCTGGACCAAAGGAGAATAACGCGGCCTGTTATTTTAACCGCGCCGAATTCACCGACGATACCTCGATGGCGCTGTGTCTGGCGGATGCGTTACTg  <  1:146416/251‑1 (MQ=255)                                                                                                                                                                                  gTTGACACAAGGGAAATTTAAATGAAAACAGAGCGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGa                                                                                                                                                                              <  1:63548/71‑1 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                                      GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAAGCACACTTTGGCTGGATTGACCGTTTTCTTCCTGGACCAAAGGAGAATAACGCGGCCTGTTATTTTAACCGCGCCGAATTCACCGACGATACCTCGATGGCGCTGTGTCTGGCGGATGCGTTACTG  >  NC_000913/2179897‑2180316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑