Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,178,654	G→A	intergenic (‑90/‑167)	fbaB ← / → yegT	fructose‑bisphosphate aldolase class I/putative transporter YegT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,178,654	0	G	A	100.0%	30.2 / NA	12	intergenic (‑90/‑167)	fbaB/yegT	fructose‑bisphosphate aldolase class I/putative transporter YegT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

ATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTACCTGTCATGAAGGAATTAAAAGATGAAAACAACAGCAAAGCTGTCGTTCATGATGTTTGTTGAATGGTTTATCTGGG  >  NC_000913/2178436‑2178875                                                                                                                                                                                                                           |                                                                                                                                                                                                                              attGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTGGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCa                                                                                                                                                                                                <  1:94649/250‑1 (MQ=255)                                          gaTAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAgcgc                                                                                                                                                      <  1:12306/251‑1 (MQ=255)                                                    gTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATAGCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTGTGGATGTTTTTTCAGCGTTTTCTGTTGTCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGttt                                                                                                                                            <  1:160226/251‑1 (MQ=255)                                                          aGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCAccn                                                                                                                                      <  1:15434/251‑2 (MQ=255)                                                                                                                               cATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGACTTTTGCGGGCAATCATACTCCTGGTCGTTACGGACGCGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTAATCAGAAAAAATGTTAGCGCGGTCACGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTCCCTGTCATGAAgg                                                                  >  1:91580/1‑250 (MQ=255)                                                                                                                                    cTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGtttt                                                                                                                                                                                         >  1:143060/1‑126 (MQ=255)                                                                                                                                           cccGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATtc                                                                                                                                                                          >  1:61418/1‑134 (MQ=255)                                                                                                                                                     ncGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTACCTGTCATGAAGGAATTAAAAGATGAAaac                                                 >  1:16730/2‑245 (MQ=255)                                                                                                                                                                                    cccGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCCGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTACCTTTCATGAAGGAATTCAAAGATGAAAACAACAGCAAAGCTGTCGTTCATGATGTttgttggctgg            >  1:80528/1‑246 (MQ=255)                                                                                                                                                                                    cccGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTACCTGTCATGAAGGAATTAAAAGATGAAAACAACAGCAAAGCTGTCGTTCATGATGTTTGTTGAATgg            >  1:863/1‑251 (MQ=255)                                                                                                                                                                                              gCGGGNNATNNNAANNCTGGTCGTTACGGACGAGTTTTTNCATCTTTTTGATGTTTTTTCAGCGTTTTCNGNTNGCTCNNTTCNTCAGAAAAAANGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTNANTGGTATTGTCATCGCGTACCTTACATTACCTNTCATGAAGGAATTAAAAGATGAAANAAACNNCanannnnn                                   >  2:128318/1‑213 (MQ=255)                                                                                                                                                                                              gCGGGCAATCATAATCCTGGTCGTTACGGACGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGCTTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTAACTTCCATTACCTGTCATGAAGGAATTAAAAGATGAAAACAACAGCAAAGCTGTCGTTCATGATGTTTGTTGAATGGTTTATCTggg  >  1:62871/1‑251 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                              ATTGTCAATCATTACGCGGTCTACGTAGTCATGTCCGGGGAGATAAAGCTGGTCAGAAGGAATTGTCATACAACGGTGCTGTAAAAGGTTGTCGGCGTCTTTGCCAAGCAACTGCGCAATATCTGTCATTACTATGCTCCCGTAAATTCCGATTGGATATCGGCTATGGATTGTCCTGGCCCGCCTTTTGCGGGCAATCATAATCCTGGTCGTTACGGGCGAGTTTTTCCATCTTTTGGATGTTTTTTCAGCGTTTTCTGTTGGCTCGATTCATCAGAAAAAATGTTAGCGCGGTCAAGTTTTCACCGCAAAGGTATTTAAAAGGTATTATTAAGTGGTATTGTCATCGCGTACCTTACATTACCTGTCATGAAGGAATTAAAAGATGAAAACAACAGCAAAGCTGTCGTTCATGATGTTTGTTGAATGGTTTATCTGGG  >  NC_000913/2178436‑2178875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑