Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,177,343 G→A intergenic (‑139/+169) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,3430GA100.0% 14.7 / NA 7intergenic (‑139/+169)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

AACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGT  >  NC_000913/2177145‑2177566
                                                                                                                                                                                                      |                                                                                                                                                                                                                               
aaCCGCAAAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAcn                                                                                                                                                                             <  1:15393/251‑2 (MQ=255)
   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                                                                                                                                                          >  1:86358/1‑251 (MQ=255)
                                                                                                                                                                 cGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTg                                                                                                                                                                                                                 <  1:12272/54‑1 (MQ=255)
                                                                                                                                                                 cGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTg                                                                                                                                                                                                                 >  2:12272/1‑54 (MQ=255)
                                                                                                                                                                  gaggagTGAAAGGCAAACAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTc                                                                                        >  1:40023/1‑174 (MQ=255)
                                                                                                                                                                  gaggagTGAAAGGCAAAAAANNGGCCTCCCGNTAGGAAAGCCGTAGCAAAGTGCGCGTGGTTTTATGCCGGATGCGGTGTAAACANACGTGGCAGNNCTNTGTAGNCNNNANNNGANGCTTCAGCGTCGCATCAGGNATNNGTTGCCNGATGCAGCGTAAACGCCTTATCCGTc                                                                                        <  2:40023/174‑1 (MQ=38)
                                                                                                                                                                              gCAAAAAAACGGCCTCCCGATAGGAAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGGCCTGCACGGCGTTAATCAGt  <  1:61801/248‑1 (MQ=255)
                                                                                                                                                                                                      |                                                                                                                                                                                                                               
AACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGCATAGGTTGCCGGATGCAGCGTAAACGCCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGT  >  NC_000913/2177145‑2177566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑