Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,051	G→A	L86L (CTG→TTG)	yegR ←	uncharacterized protein YegR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,051	0	G	A	100.0%	14.1 / NA	6	L86L (CTG→TTG)	yegR	uncharacterized protein YegR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/5);  total (1/5)

AGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAGCTTCGGCTTCGGTGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTG  >  NC_000913/2167879‑2168233                                                                                                                                                                             |                                                                                                                                                                                       aGAATCCTCCTTCATAGCACGATGCCGTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATGCTCACTCTCCACATGTGAATGTCAGACGAGCCACACCATGTAATCCCACACCTTCTGTCTTCAACTCAACTATTTTCATTTTTTTGCCCTGAGTAac                                                                                                                                                       <  1:153766/206‑1 (MQ=255)         cctTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTCCGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTTTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCCACACCTTCTGTCTTCAACTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCTTTTTTTACTATATTTCCTGCACCATATATTCt                                                                                                    <  1:12730/249‑1 (MQ=255)                                   aTCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCACACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAACTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAgcttcggcttcg                                                                        <  1:141247/251‑1 (MQ=255)                                                                                       tttGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAACTCAACTATTTg                                                                                                                                                                            <  1:5040/99‑1 (MQ=255)                                                                                       tttGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAACNNAACTAtn                                                                                                                                                                              >  2:5040/1‑96 (MQ=255)                                                                                                         atatTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAACTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAATCTTCGGCTTCGGTGTATGATTGGTTATCTAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACttn  <  1:16276/251‑2 (MQ=255)                                                                                                                                                                             |                                                                                                                                                                                       AGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACGCACAGCCAGAACGCGCATATTTGACGCTTACCAAAAAATATTCTCACTCTCCACATTTGAATGTCAGACGAGCGACACCATGTAATCCTACACCTTCTGTCTTCAGCTCAACTATTTGCATTTTTTTGCCCTGAGTAACACAGAAATGAGTTGCATCATTTTTTACTATATTTTCTGCACCAGATATTCTACCCCTGGCTAAAGAAGCTTCGGCTTCGGTGTAGTATTGGTTATCGAGTTTACGCTGAATATTACTTTTATATGCAAGACCAAATTTACCGATACTTG  >  NC_000913/2167879‑2168233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑