Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,216	G→A	S985N (AGT→AAT)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,216	0	G	A	88.9%	15.3 / ‑5.8	9	S985N (AGT→AAT)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (2/6);  total (2/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

ATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCC  >  NC_000913/2157993‑2158445                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      aTTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGATCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCTCTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGcgtcg                                                                                                                                                                                                            <  1:99898/251‑1 (MQ=255)                                          tattTTGCTGATCTGTATCGTGAAGAAGAACGCCATCATGATGATCGACTGCGCGGTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCGGGCGTTTCTGTTGCGGTTTCGTCTGATCCTGATGACCTCTCTGGCGGCTCGTCTTGGCGCGCTTCGTGTGAGGTCGAGTACCTGGGTCGGCTCGGAACTGCTTCGTCCGTGAGGTATCTGCATGGTCGGCCGTCTGATTGTCatc                                                                                                                                                                    <  1:157717/250‑3 (MQ=255)                                                                                                                                cgcgcgAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGTGGTCTGATTGTCAGCCAGGAGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCAt                                                                             >  1:172509/1‑251 (MQ=255)                                                                                                                                          aaTCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGTCGTCCGTGAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGTTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGaggagg                                                                   <  1:21448/251‑1 (MQ=255)                                                                                                                                                                   tttCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATGTGCTGTTCGan                                                                                                                    <  1:40956/177‑2 (MQ=255)                                                                                                                                                                      cGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCGGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGGCGTCCGTTAGGTAGCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGGTTGCCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGc                                                                                            <  1:76064/198‑1 (MQ=255)                                                                                                                                                                       gTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATTGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTGCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTg                                                 <  1:169724/240‑1 (MQ=255)                                                                                                                                                                                               ttgcgcttccgatctcgcTGCCGCTGATGTTTAATACCGGGGTCGGCGCGGACCTGCGTCTTCCTTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGATCTGACGCTTTTTCCCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTTTGGGCCAAAAGCCGCTTTGCCCGTCATGAAGATTATGCTTTAGTTAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGAtttt               <  1:101209/235‑1 (MQ=255)                                                                                                                                                                                                            cgcgcTGCCGCTGATGTTGAATACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGAAGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTACGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGcc  >  1:64134/1‑250 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                      ATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCC  >  NC_000913/2157993‑2158445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑