Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,156,361	G→A	A367T (GCG→ACG)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,156,361	0	G	A	100.0%	12.5 / NA	8	A367T (GCG→ACG)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/2);  total (6/2)

TCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCGGCACTTTCGCGGTTATGGTGTTTCTCGATTTTTCAATCAATAACCTGACACTGATGGCGTTAACTATCGCCACCGGATTCGTGGTCGATGACGCCATCGTGGTGATCGAAAACATTTCCCGCTATATCGAAAAAGGCGAAAAACCGTTGGCGGCGGCGCT  >  NC_000913/2156144‑2156563                                                                                                                                                                                                                          |                                                                                                                                                                                                           tCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTg                                                                                                                                                                            <  1:5138/250‑1 (MQ=255)           nccGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGAAGATGCTGTAACAGCTCACTGAGAGTCTGCCGCAATCGGTGCAGGAGACAGTGCTTTCCGATCGCGCCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCAGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCgg                                                                                                                                                                 >  1:15882/2‑251 (MQ=255)                         tATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCCCACTGAGAGACTGCCGCCATTAGTGAACGAGCCAGGGCTTTCCGATCGCCCCACCAATATCCGCGCATCCGTCGATGATACTCAGCTTGCATTGATGATGGCTATCGCGCTGGTAGTCGTGATTATCTACCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTAAATCGGCACTTTCGCGGtt                                                                                                                                                    >  1:70943/1‑250 (MQ=255)                                                                                         gTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCGGCACTTTCGCGGTTATgg                                                                                                                                                >  1:80468/1‑190 (MQ=255)                                                                                                                                 gcgcATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGCCGTCATGCTTCTCTCCCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTCATCGGCACTTTCGCGGTTATGGTGTTTCTCGCTTTTTCACTCAATAAcc                                                                                                                     >  1:123238/1‑177 (MQ=255)                                                                                                                                            gatgatACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCGGCACTTTCGCGGTTATGGTGTTTCTCGATTTTTCAATCAATAACCTGACACTGATGGCGTTAACTATCGCCACCGGATTCGTGGTCGATGACGCCATCGTGGTGATCGAAAACATTTCCCGCTATATCg                                 >  1:46700/1‑250 (MQ=255)                                                                                                                                                                          aTCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCACTATTCCGACGACCATCATTCCCGGTGTTGCTGCACAGCTCTCGTTACACGGCACTTACGCGGTTATGGTGTTTCTCGATTTTTAAATCCATAACCTGAAACTGCTGGTGTTAACTATCGCCACCGGATTCGTGGTCAATGACGCCATCGTGGTGATCGAAAACATTTCCCGCTATATCGAAAAAGGCGAAAAACCGCTGTCGACGGCGCt  >  1:169277/1‑251 (MQ=255)                                                                                                                                                                                  ggTAGTCATGATGATCTACCTGTTTTTGCGCAATATTCCGACGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCGGCACTTTCGCTGTTATGGTGTTTCTCGATTTTTCAATCAATAACCTGACACTGATGGCGTTAACTATCGCCACCGGATTCGTGGTCGATGACGCCATCGTGGTGATCGAAAACATTTCCCg                                         <  1:22017/204‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                           TCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCATGATTATCTACCTGTTTTTGCGCAATATTCCGGCGACCATCATTCCCGGTGTTGCTGTACCGCTGTCGTTAATCGGCACTTTCGCGGTTATGGTGTTTCTCGATTTTTCAATCAATAACCTGACACTGATGGCGTTAACTATCGCCACCGGATTCGTGGTCGATGACGCCATCGTGGTGATCGAAAACATTTCCCGCTATATCGAAAAAGGCGAAAAACCGTTGGCGGCGGCGCT  >  NC_000913/2156144‑2156563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑