Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,152,528	G→A	R201C (CGC→TGC)	yegL ←	IPR002035 domain‑containing protein YegL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,152,528	0	G	A	100.0%	16.2 / NA	7	R201C (CGC→TGC)	yegL	IPR002035 domain‑containing protein YegL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

TCATTGACGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCAC  >  NC_000913/2152282‑2152734                                                                                                                                                                                                                                                       |                                                                                                                                                                                                               tCATTGACGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGa                                                                                                                                                                                                             <  1:54125/250‑1 (MQ=255)                                                                                       gTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGc                                                                                                                                             <  1:138210/228‑1 (MQ=255)                                                                                                                               cAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGc                                                                                                                   >  1:55911/1‑214 (MQ=255)                                                                                                                                                                                                           nccTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGAAACGGAACGAAGCGAACTGGATAACAAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTCATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCAc  >  1:30932/2‑251 (MQ=255)                                                                                                                                                                                                           gCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGAAACCGACCGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCATTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTg     >  1:68291/1‑248 (MQ=255)                                                                                                                                                                                                                   gcgcTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCAGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTGCATATCCGCCCCCGGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGc                         <  1:67890/220‑1 (MQ=255)                                                                                                                                                                                                                   gcgcTNCCAGCNCNNCTTCCGTTCCCGGCGTGGANCAGGAAACCGAACGCNGCGANCTGGANAAnc                                                                                                                                                                                   >  2:67890/1‑66 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                               TCATTGACGGCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTGTAATCCTTGCAGAGGCAAAGGCTGACGAACGCTAATTTGTGCCAGCGTCTTCATATCCGCCCCCTGAACACCGATGGAAAAGAAAGCAAACCTTTTATCTTCTTCCCCCCGAAACACTTTGTTGGCTGCAGCCTGCCACTCATCGGTTGGTGCAC  >  NC_000913/2152282‑2152734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑