Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,524	G→A	D114N (GAT→AAT)	yegJ →	DUF2314 domain‑containing protein YegJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,524	0	G	A	100.0%	16.2 / NA	7	D114N (GAT→AAT)	yegJ	DUF2314 domain‑containing protein YegJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

GCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC  >  NC_000913/2151284‑2151734                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                    gCGATGTCTGACGAGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATAACTAAAAAAGATGCCGATCCTTTCGCAGTCAAAGCCCCGATCACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACATATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGGAAGAAAATATTTc                                                                                                                                                                                                              >  1:129717/1‑248 (MQ=255)      ntCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTACTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATAAAGAAAGAAAATATTTAAGACTgg                                                                                                                                                                                                       >  1:35127/2‑250 (MQ=255)                                   aaaCCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAAATATGTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTAcggc                                                                                                                                                                        <  1:147548/251‑1 (MQ=255)                                                                                                                                              gTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATTGCCAGGAGTGGAAAATCAAGAAATAAAATATTTTAGACTGGA‑TGTATACACGCGGGGAAANAATNNNnngc                                                                                                                                                                        >  2:106273/1‑144 (MQ=255)                                                                                                                                              gTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAAATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTAcggn                                                                                                                                                                        <  1:106273/144‑2 (MQ=255)                                                                                                                                                                                          cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAAATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGc                                                                                                                         <  1:99452/147‑1 (MQ=255)                                                                                                                                                                                                         aaaCGAAGAAGATGCCCAGAAGTGGAAAATGAAGAAAAAAAATATTCCAGAC‑GGAGTGTATACGCGCGGGAATAAAAATTCTGGATGTTATACCATTGATCCGTTATTTGCCACCTTTCCAAAAAAGGAGGCGGGGGAGCAAAGAGCAAAATTAATCCCCTAATGATCTGTTATATTTTTTTTTTAAATGGGCTTTTGAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc  <  1:83962/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                    GCGATGTCTGACGCGATTAAACATGCGCGGAAAGAAACCGATAAATTCATCGAGGTTATGAATAAAAAAGATGCCGATACTTTCGCAGTCAAAGCCCCGATAACCGATCACGGAAGAACCGAGCATTTCTGGCTTACGGATGTCACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGA‑TGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGC  >  NC_000913/2151284‑2151734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑