Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,138	G→A	F616F (TTC→TTT)	yegI ←	putative serine/threonine‑protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,138	0	G	A	100.0%	27.2 / NA	10	F616F (TTC→TTT)	yegI	putative serine/threonine‑protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/7);  total (3/7)

AGAACAGTTGCCGGGCATTCCGATTGCAGGCGGCGATGACTTTGGCTCCGTCACCGCCGGGCTGGCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGGAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCG  >  NC_000913/2148933‑2149291                                                                                                                                                                                                              |                                                                                                                                                          aGAACAGTTGCCGGGCATTCCGATTGCAGGCGGTGATGTTTTTGGTTCCGTCACCGCCGGGTTGGCACGCTGGGCGGAAGTTGTGGTTCGTTAATTGAAAAAGGGCTAAGCAGCGGCTTAAATTTGCCTTCGCTGGAGTCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGc                                                                                                              <  1:92541/251‑1 (MQ=255)              ggCATTCCGATTGCAGGCGGCGATGACTTTGGCTCCGTCACCGCCGGGCTGGCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTgg                                                                                                 <  1:27892/251‑1 (MQ=255)                                     tGACTTTGGCTCCGTCACCGCCGGGCTGGCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTGTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGc                                                                                                <  1:118590/229‑1 (MQ=255)                                             gCTCCGTCACCGCCGGGCTGGCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTtgtctgtc                                                                  <  1:138608/251‑1 (MQ=255)                                                                ggCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTcg                                                                                                                                        <  1:142862/162‑1 (MQ=255)                                                                        gggCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTc                                                                                            <  1:153457/198‑1 (MQ=255)                                                                                                              ncagcGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCg  >  1:108562/2‑250 (MQ=255)                                                                                                                         aaTCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGc                                                   <  1:134300/190‑1 (MQ=255)                                                                                                                                ctgcGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCtt                                                 >  1:160099/1‑185 (MQ=255)                                                                                                                                                       ctgcCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGAAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCCGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACgg                          >  1:157173/1‑185 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                          AGAACAGTTGCCGGGCATTCCGATTGCAGGCGGCGATGACTTTGGCTCCGTCACCGCCGGGCTGGCACGCTGGGCGGAAGTGGTGTTTCGTTAATTGAAAAATGGCTCAGCAGCGGCTTAAATCTGCCTGCGCCTGAGCCAGTTTTTCTGCTGCCTGACGTAACGGTTCCATCAACGGCATGGTCCGTGCTGGAGCATGAAGGCGGAATCGCTGCAACTCTGTCGCGCCGACTGTCAACGCCGATTCCAGCCGATGTCGTTTGGCGGTCATTTTCGCCATTACCGCTTGTCTGTCTGCTGGCGTTATCGCTTCATTCGGCCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCG  >  NC_000913/2148933‑2149291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑