Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,050,275	G→A	D1780N (GAC→AAC)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,050,275	0	G	A	100.0%	16.7 / NA	8	D1780N (GAC→AAC)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGAAACGGTTCGTATATGGTGAAAGCATCCCTGCCGAATGGAGCCTCACTTGAGAAACAACTGGAGGCTATTGATGAAAAACTGAC  >  NC_000913/2050041‑2050519                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     gTTAATTTCAGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGATGAAACGGATGATCGGGGTTTCGCTGAGATTCTTGTGACAAGAACCGAGGACGGAATGAAAACAGTTTAAGCCTCTCTGGCAGATAAACCTCCTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTACGACGATTACCAGTCTGGAGATACCGGCAGATCAGGTAATGGTCGCACAAAACGTAACAGTTaacgc                                                                                                                                                                                                                                      >  1:29369/1‑248 (MQ=255)                                           gTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAAGGGTCGCACAAAACGGAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAAcc                                                                                                                                                                                            <  1:84427/251‑1 (MQ=255)                                                                                                                               gATAAACCTCCTGAAGCCATCGCGCGATGCCTGAATGCCAGTGCAGATGTTAATTCTGCGACGAGTTCCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAAACGTAGCAGTTAAAGCTCACGTTAACGCCCAGTTTGGCAAACCGGTTgc                                                                                                                                                                                                     <  1:168582/158‑1 (MQ=255)                                                                                                                                                           tACTGAATGCCAGTGCCGACGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAAACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGACACGGTTCg                                                                            >  1:59603/1‑251 (MQ=255)                                                                                                                                                                               gTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAAACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGAAACGGTTCGTATATGGTGAAAGCATccc                                                         <  1:71897/250‑1 (MQ=255)                                                                                                                                                                                             gaTTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAAACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACACGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGAAACGGTTCGTATATGGTGAAAGCATCCCTGCCGAATGGAGCCt                                          >  1:98248/1‑251 (MQ=255)                                                                                                                                                                                                                           ggTAATGGTCGCACAAAACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGCTCATCAGCCAGAATACGGTCTCTNCTANNNNNCannnnnnnnnn                                                                                                             >  2:107489/1‑144 (MQ=255)                                                                                                                                                                                                                                      nacaAAACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGAAACGGTTCGTATATGGTGAAAGCATCCCTGCCGAATGGAGCCTCACTTGAGAAACAACTGGAGGCTATTGATGAAAAACTGac  >  1:18870/2‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     GTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTGGCAACCCGGTTGCGCATCAACCCGTGACATTCAGTGCAGAGCCATCCTCGCAAATGATCATCAGCCAGAATACGGTCTCTACTAATACGCAGGGTGTAGCCGAGGTCACCATGACGCCCGAAAGAAACGGTTCGTATATGGTGAAAGCATCCCTGCCGAATGGAGCCTCACTTGAGAAACAACTGGAGGCTATTGATGAAAAACTGAC  >  NC_000913/2050041‑2050519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑