Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,053	G→A	A185V (GCT→GTT)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,053	0	G	A	100.0%	37.1 / NA	14	A185V (GCT→GTT)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/8);  total (6/8)

ATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTG  >  NC_000913/2002812‑2003216                                                                                                                                                                                                                                                  |                                                                                                                                                                    aTTTGCCTTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTGCCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTATTTCCGCCAGTATCAGTGGCTGCGTCCGGAGAAAGGGGAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTgg                                                                                                                                                            <  1:127945/251‑1 (MQ=255)                          gTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTGCCGCCAGTAGCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGGTGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAActc                                                                                                                                    <  1:87343/250‑3 (MQ=255)                                                     aCTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGc                                                                                                        <  1:90261/251‑1 (MQ=255)                                                      ntGCGTAATACTTCCCATCGTTCTCAACACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTa                                                                                                                                                          >  1:139172/2‑200 (MQ=255)                                                                                                    gTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGGAATTCAAGTAAGTTTAATATTGTTTGTGGGGGTAGCACCAAAAGCAGTTACTGGAACACTCATGGTAACTGGCTCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGGGATAGTCTGGTTCTCATTTgcgc                                                         >  1:89981/1‑251 (MQ=255)                                                                                                            tACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCTCCAGTATCAGTGGCTGCGTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTAGCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTg                                                  <  1:149498/250‑1 (MQ=255)                                                                                                                       gTATAAACACCATCAATTGAAGCTGGGTTAGTTCCGACAGTATCAGTGGGTGCTTCCGTAGAAAGGGCAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATCTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTg                                                            >  1:168488/1‑229 (MQ=255)                                                                                                                                    cAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCGGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCTGTATTTTTAACTCTAAAACCAGCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTg                           <  1:10605/249‑1 (MQ=255)                                                                                                                                               gggTTAGGTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATGCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTGACTGGAACTCTAGTGGGAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCGTCAGATCGATAGTGATAGTCTGGTTATCAGTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCAc              <  1:72297/251‑1 (MQ=255)                                                                                                                                                 gTTAGTTCCGCCAGTATCAGTGGCTGCTTAAGTAGAAAGGGTAATTCCAGGAAGTTTACTATTGTTTGTAGAGGTCGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACATGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGc            >  1:152060/1‑251 (MQ=255)                                                                                                                                                     gTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACGGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAATAGTTGTAGCATCAATCTGCTGCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTg                           <  1:162838/232‑1 (MQ=255)                                                                                                                                                           ccAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACAAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGcaatcggaagagcacacgtctgaac  >  1:120469/1‑227 (MQ=255)                                                                                                                                                                                nnaGNNNNNGTANTTCCAGTAAGTATAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATc                                                                                      <  2:102806/144‑1 (MQ=255)                                                                                                                                                                                       gggTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAACACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCa                                                                                                                         >  1:45737/1‑104 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                    ATTTGCCGTTGCTCCAGTCGCCATTGTCACTGTACCATCATTAGCAACTGTTACTGCGTAATACTTCCCATCGTTATCACCACCGGTGATTTTCGCATAGTAATCATTACCATTATCAGTATAAACACCCTCAATTGAAGCTGGGTTAGTTCCGCCAGTATCAGTGGCTGCTTCCGTAGAAAGGGTAATTCCAGTAAGTTTAATATTGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTG  >  NC_000913/2002812‑2003216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑