Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,980,601	G→A	Q338* (CAG→TAG)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,601	0	G	A	100.0%	34.8 / NA	13	Q338* (CAG→TAG)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

GCAACTTCGTCACGATCGTAGGGGTTAACAATTAACGCCGACGTTAACTCGTTTGCCGCTCCCGCAAATTGCGAAAGAACAAGAACGCCCGGATTGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGA  >  NC_000913/1980384‑1980791                                                                                                                                                                                                                          |                                                                                                                                                                                               gtaaCTTCGTCACGATCGTAGGGGTTAACAATTAACGCCGACGTTTACTCGTTTGCCGCTCCCACAAATTGCGAAAGAACAAGAACGCCCGGATTGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGAGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTg                                                                                                                                                               >  1:47570/3‑251 (MQ=255)                         ttAACAATNANNNCNNCCGTTAACTCGTTTGCCGCTCCCGCAAATTGCGAAAGAACAAGANCGCCCGGATTGGCTGNGTNCTGANCAGNAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCCCTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTANTTCNAATAANNANACGGAGNNNAgnnnnnnnntnnnnatnnc                                                                                                                                                     >  2:119216/1‑219 (MQ=255)                              aaTTAACGCCGACGTTAACTCGTGTGCCGCTCCCTCAAATTGCGAAAGACCAAGAACGCCCGGATGGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAGTTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATGAATTCGTTCAGCtt                                                                                                                                  <  1:38752/251‑1 (MQ=255)                                        gACGTTAACTCGTTTGCCGCTCCCGCAAATTGCGAAAGAAGAAGAACGACCGGATTGGCTGGGTCCTGAGAAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGACCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGATAATTCAAATAATAAAGCGGCGTCCAGCCTACTTGCACGTATTTACCATTAATTCGTCCAGCTTCATTTTCg                                                                                                                          >  1:158328/1‑249 (MQ=255)                                        gACGTTAACTCGTTTGCCGCTCCCGCAAATTGCGAAAGAACAAGAACGCCCGGATTGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCGAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAg                                                                                                                        <  1:47171/251‑1 (MQ=255)                                                                                         ccGGATTGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCANc                                                                                                                                    <  1:134615/190‑1 (MQ=255)                                                                                                                  aaCATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGTCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGCCAAAATGCTAACTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTGACCATTAATCCGTCTAGTCTCATTTTCCAGGTGATGACGAACGGCCTGAGAGGTTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAAttttcc                                              <  1:164922/251‑3 (MQ=255)                                                                                                                                                        gTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATGTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCAACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAg        <  1:175217/251‑1 (MQ=255)                                                                                                                                                            cGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATcn                                                                                                        <  1:20860/151‑2 (MQ=255)                                                                                                                                                               cACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGACAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCgaga  >  1:72296/1‑250 (MQ=255)                                                                                                                                                                                    gCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAAt                                                                                                                                             <  1:94651/90‑1 (MQ=255)                                                                                                                                                                                      ggAATATTTTCATCAGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATc                                                                 >  1:4849/1‑164 (MQ=255)                                                                                                                                                                                                catcaGTAATTTACGGTCAAAATGCTAATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTg                                                                      >  1:157563/1‑149 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                               GCAACTTCGTCACGATCGTAGGGGTTAACAATTAACGCCGACGTTAACTCGTTTGCCGCTCCCGCAAATTGCGAAAGAACAAGAACGCCCGGATTGGCTGGGTCCTGAGCAGCAACATACTCTTTTGCTACCAGGTTCATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGA  >  NC_000913/1980384‑1980791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑