Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,971,999	G→A	A231A (GCC→GCT)	tar ←	methyl‑accepting chemotaxis protein Tar

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,971,999	0	G	A	100.0%	15.7 / NA	7	A231A (GCC→GCT)	tar	methyl‑accepting chemotaxis protein Tar
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

TCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTGACGATATCGCGATACAGTTTTTCACTGCTGAGGGCGTACTGAGCAAACGCTTCGCCCATTGC  >  NC_000913/1971769‑1972214                                                                                                                                                                                                                                       |                                                                                                                                                                                                                        cccATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCATTACGGGAGGAAAGATCGGTGGTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTTAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGTTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGTTATTCGCCATGTTACCACCAGCGATTTCGCGAATGTGAGc                                                                                                                                                                                                     <  1:161771/250‑1 (MQ=255)                                                                            gcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGCAGGTGTCCGTCCAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCAGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATaccaccac                                                                                                                          >  1:23724/1‑251 (MQ=255)                                                                                                                                           gtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACAACCAGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTAACACGCCACCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTGACGat                                                           >  1:25896/1‑251 (MQ=255)                                                                                                                                                                                   cGCCCATTTCCCTGCTCCCGTCAATGGTCAGGGTATTCGCCATGTTCCCACCAGCGATTTCGCGAATGTGAGCAATACTTTTTGCCAGGGGAGTAAGCAACATACGGCGAATGCCGGACCACGCCCCCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTGACGATATCGCGATACAGTTTTTCACTGCTGAGGGCGTACTGAGc                    <  1:2053/250‑1 (MQ=255)                                                                                                                                                                                                  ncccGTCAATGGTCAGGGTATTCGCCAGGTTACCACCAGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATACCACCACCAGCGCGa                                                                                                                  >  1:139087/2‑141 (MQ=255)                                                                                                                                                                                                     ngTCAATGGTCAGGGTATTCGCCAGGTTACCACCAGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTAGCACGCCACCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTGACGATATCGCGATACAGTTTTTCACTGCTGAGGGCGTACTGAGCAAACGCTTCGCCCAttgc  >  1:107362/2‑250 (MQ=255)                                                                                                                                                                                                        cAATGGTCAGGGTATTCGCCAGGTTACCACCAGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGGGGAGGAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTTACGATATCGCGATACAGttttt                                          <  1:46773/207‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                        TCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATACCACCACCAGCGCGATAACCGCCAGTTGCCACTGGGCAAATCGGTAATCATCTGCGTTGTCAGTGACGATATCGCGATACAGTTTTTCACTGCTGAGGGCGTACTGAGCAAACGCTTCGCCCATTGC  >  NC_000913/1971769‑1972214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑