Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,959,049	G→A	A73V (GCC→GTC)	cutC ←	PF03932 family protein CutC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,959,049	0	G	A	100.0%	15.5 / NA	8	A73V (GCC→GTC)	cutC	PF03932 family protein CutC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

GCCCTGATGTCAGTACCCGGGCAATGCCCAATTCCGCAAGATTATTGAGTGTATATAAAGGGTTAGCGCACATATCGAAGGCGCGATGAAAAGTCACTGCCAGCGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACATTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGCGCACATCCTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAAGCCCCCCTCTTTTGGGGCTGCGCATAATTCAACTCTGTCTGCGCCGTTTTGCTGCGCCGTTAGTGCACATTCCATGCTGTAACAGCAAATTTCCAGTAATGCCATTTTTAC  >  NC_000913/1958819‑1959272                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                gCCCTGATGTCAGTACCCGGGCAATGCCCAATTCCGCAAGATTATTGACTGTATATAAAGGGTTAGCGCCCCTATCGAAGGCGCGATGAAAAGTCACTGCCAGCGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACTTTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGCGCACATCCTCAAGAATGGCGACAAACTCACCGTCGCTGt                                                                                                                                                                                                               >  1:170103/1‑249 (MQ=255)  cccTGATGTCAGTACCCGGGCAATGCCCAATTCCGCAAGATTATTGAGTGTATATAAAGGGTTAGCGCACATATCGAAGGCGCGATGAAAAGTCACTGCCAGCGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACACTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGCGCACATCCTCAAGAATGGCGACAAACTCCCCGTCGCTGTaa                                                                                                                                                                                                             >  1:168441/1‑249 (MQ=255)                           cccAATTCCGCAAGATTATTGAGTGTATATAAAGGGTTAGCGCACATATCGAAGGCGCGATGAAAAGTCACTGCCAGCGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACATTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGTGCACATCCTCAAGAATGGCGACAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAAt                                                                                                                                                                                   >  1:58011/1‑251 (MQ=255)                                                                                                        cGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACATTCCCGCCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGGGCGCACATCCTCAAGAATGGCGACAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAAGCCCCCCTCtt                                                                                                      >  1:965/1‑251 (MQ=255)                                                                                                                    agcCATTATTTTTTCCATTCGTGGCATATCGACATTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGCGCACATCCTCAATAATGGCGACAAACTCACCTTCGCTGTATCAAAAATCACCACCGCGTTGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTTGCGCACGTATTTCAGTACACCCAACGACGGCGTTAAGCCCCCCTCTTTTGGGGCTgcgc                                                                                          <  1:100082/251‑1 (MQ=255)                                                                                                                                    aTTCGTGGCATATCGACATCCCCGTCAGCATCGAGAACGCCCGTCACCAGTCCAGTAAAACCTAATTCGCGGACCGTGCGCACATCCTCAAGAATGGCTACAAACTCACCGGCGCTTTAGCAAAAATCACCACCGCCTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTg                                                                                                                                                     <  1:171130/176‑1 (MQ=255)                                                                                                                                                                cATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAAGTCGCGGACCGTGCGCACATCCTCAAGAATGGCGACAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTAGCGGATGCACAGGTATCGTCACCCGCTGGCGCACGGAGTTCAGTACACCCAACGACGGCGTGAAGCCCCCCTCTTTTGGGGCTGCTCATAATTCAACtctg                                                                            <  1:59880/221‑1 (MQ=255)                                                                                                                                                                                                            aCCGTGCGCACATCCTCAAGAATGGCGACAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAAGCCCCCCTCTTTTGGGGCTGCGCATAATTCAACTCTGTCTGCGCCGTTTTGCTGCGCCGGTAGTGCACATTCCATGCTGTAACAGCAAATGTCCAGTAATGCCATTTTTAc  <  1:58515/251‑1 (MQ=255)                                                                                                                                                                                                                      caTCCTCANNAANNGNGNCAAACNCACCGTCGCTGTAGCAAAAATcac                                                                                                                                                                                                   >  2:116663/1‑48 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                GCCCTGATGTCAGTACCCGGGCAATGCCCAATTCCGCAAGATTATTGAGTGTATATAAAGGGTTAGCGCACATATCGAAGGCGCGATGAAAAGTCACTGCCAGCGGACCGGCAGCAGCCATTATTTTTTCCATTCGTGGCATATCGACATTCCCGTCAACATCGAGAACGCCCGTCACCAGTCCAGGAAAACCTAATTCGCGGACCGTGCGCACATCCTCAAGAATGGCGGCAAACTCACCGTCGCTGTAGCAAAAATCACCACCGCGTGGGCGAATTATCGGATGCACAGGGATCGTCACCCGCTGGCGCACGGATTTCAGTACACCCAACGACGGCGTTAAGCCCCCCTCTTTTGGGGCTGCGCATAATTCAACTCTGTCTGCGCCGTTTTGCTGCGCCGTTAGTGCACATTCCATGCTGTAACAGCAAATTTCCAGTAATGCCATTTTTAC  >  NC_000913/1958819‑1959272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑