Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,433	G→A	A67V (GCT→GTT)	nudB ←	dihydroneopterin triphosphate diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,433	0	G	A	100.0%	18.6 / NA	9	A67V (GCT→GTT)	nudB	dihydroneopterin triphosphate diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

ACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTG  >  NC_000913/1948196‑1948647                                                                                                                                                                                                                                              |                                                                                                                                                                                                                       aCAAACTGTTCAATCGCCTGCCGGTTCCTACAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAACTTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAACGACAACATCAAt                                                                                                                                                                                                           >  1:45609/1‑251 (MQ=255)                                                                                                                   ggAAGCGCAAGACAGAACCATGATTCCGTATTACGCGTAACGCCCGGCGAATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGAGCGCTGACAGTCACTTAAGGTCAGTTGTTCAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTACACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCa                                                                                          >  1:151218/1‑250 (MQ=255)                                                                                                                    gtgtgctcttcgattcccATGATTCCGTATTACGCGACACGCCCGGCGAATAGCGATGACGTAAATGTGAAAAAATTTCAACCTCTACCTTGCGCTGAAAGTCAATTAAGGTCAGTTGTCCAACGACACCATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAg                                                                                         <  1:6779/234‑1 (MQ=255)                                                                                                                          cAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGt                                                                                  >  1:69759/1‑251 (MQ=255)                                                                                                                                              gTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAACTGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGATAAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAg                                                                                                                         >  1:25451/1‑192 (MQ=255)                                                                                                                                                    cgcgTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGATGACCAc                                                        <  1:1517/251‑1 (MQ=255)                                                                                                                                                                ggCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAACGACAACATCAATGGTGCCCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGATGACCACTAAGATCGAAAc                                            >  1:76155/1‑251 (MQ=255)                                                                                                                                                                                                    aaCTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGCTCAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGc                                                                                                                                                                             >  1:128116/1‑86 (MQ=255)                                                                                                                                                                                                          aCCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAACGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCCCCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTg  <  1:3866/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                       ACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGATTCCGTATTACGCGTCACGCCCGGCGCATAGCGATGACGTAAATGTGAAAAAATTTCAAACTCTACCGTGCGCTGACAGTCAATTAAGGTCAGTTGTTCAGCGACAACATCAATGGTGACCTCTTCCTTTACTTCGCGCATGGCAGCTTGCGGCGCGGTTTCACCCTCTTCCACGCTGCCGGTTACCGACTGCCAGAAATCGGGATCGTCACGCCGCTGCAACATCAGCACCCGTTTCGTATCTTGTGCGTAGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTG  >  NC_000913/1948196‑1948647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑