Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,685	G→A	A281T (GCA→ACA)	yeaX →	carnitine monooxygenase subunit YeaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,685	0	G	A	100.0%	21.7 / NA	9	A281T (GCA→ACA)	yeaX	carnitine monooxygenase subunit YeaX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/2);  total (7/2)

CCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCTGGTGCTTGCCCGTTCCGGAAAAGAGTTTGTGGTGCCGGAAGAGATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGG  >  NC_000913/1886491‑1886928                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ccgccgATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACACTGGTGCTTGACCGTTCCGGAAAAGAGTTTGTGGTGCCGGAAGAGATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGt                                                                                                                                                                                             >  1:94489/1‑251 (MQ=255)                                                   gATGCATTTACCCTGGTGCTTGCCCGTTCCGGAAAAGAGTTTGTGGTGCCGGAAGCGATGACTATTTTGCAGGTTATTGAAAATAATAAAGACGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATAATGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGAGAAGGGTAAACg                                                                                                                                           >  1:128543/1‑251 (MQ=255)                                                                                 ggAAAAGCGTTTGTGGTGCCGGAAGAGATGACTATTTTGCAGGTTATTGTAAGTAATTACGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGCTATGCGGAACCCGCGCAACAACAATACTGGAAGGTGAAGCTGACCATTGGGGTCCATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGAGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGa                                                                                                                >  1:98370/1‑248 (MQ=255)                                                                                               ggtgCCGGAAGAGATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTg                                                                                               >  1:61478/1‑251 (MQ=255)                                                                                                    nggAAGAGATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAgcgc                                                                                          >  1:31729/2‑251 (MQ=255)                                                                                                         gagaTGTCTATTTTGCAGGTTATTGAACATACTAAAGCCGCGAAAGAGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAAAAATACTGGCAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGCAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGccc                                                                                      >  1:146832/1‑250 (MQ=255)                                                                                                              gACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTcc                                                                                 <  1:1207/250‑1 (MQ=255)                                                                                                                                              ccGCGAAAGTGGAATGTTGATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGGGCCAGGCAGCAAAGTAGGTTGATCTGTTGTTCGCGTGCGAAGGGGAAGCGCCTGGTTTTGGATTTGTAGTTTGCCGAAGCCGGATGTGTCGCTGAGCGCGCCCTGTCCGGCTACGGAAGATTTTACTGCGGATATTCCTGc                                                <  1:109556/251‑1 (MQ=255)                                                                                                                                                                                            cGAAACAACAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGACAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGCTCTTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCgg  >  1:149173/1‑251 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CCGCCGATACGCTGCACTTTGAGCAATTTGCTATCGAAGACAAAACCGGCGATGCATTTACCCTGGTGCTTGCCCGTTCCGGAAAAGAGTTTGTGGTGCCGGAAGAGATGACTATTTTGCAGGTTATTGAAAATAATAAAGCCGCGAAAGTGGAATGTTTATGTCGTGAAGGGGTATGCGGAACCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGG  >  NC_000913/1886491‑1886928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑