Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,800,738	G→A	R611R (CGC→CGT)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,800,738	0	G	A	100.0%	11.1 / NA	7	R611R (CGC→CGT)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

CCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGA  >  NC_000913/1800547‑1800973                                                                                                                                                                                                |                                                                                                                                                                                                                                            ccTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGACGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCatat                                                                                                                                                                                   >  1:114671/1‑250 (MQ=255)       aGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGACGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGgac                                                                                                                                                                            <  1:38957/251‑1 (MQ=255)                  gCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGATTGAAATCGTTTTACGCCTTTCATACATTCTTCCTCCAATTGCTTACGACTGCGCCTGAGAATCTCTTGTTGCAGCTTCTCGATCCCTTCATTTACGTACATGCTTCCCAGGTCTTTACCACGACGGGTGCGAACGGACACTTTGCCTGATTCAACCTCTTTATCACCACATACCAGCatacgcccctccggggagagcg                                                                                                                                                                 >  1:162280/1‑232 (MQ=255)                                                           ggCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCAGGCTTCCCAGGTCTTTACCACGACGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCCCCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAg                                                                                                                        <  1:6584/251‑1 (MQ=255)                                                                                                                                                                        cATGCTTCCCAGGTCTTTACCACGACGGGTGCGAACGGCAACTTTTCCTGATTCCTCCTCTTTATCACCACGGACCAGCATATATGGGACGCGACGCAAAGTATGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCGTCTTTAACACGATTGCCGGCGTTTGATAGGTTTTGCGGCAATTCGTTAACGTATTCAGACTGTGTTTCTGTAATATTCATGATAACAACCTGAACCGGCGCAAGcc           <  1:173503/251‑1 (MQ=255)                                                                                                                                                                                 cAGGTCTTTACCACGAGGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAATTCTGCGTGAACACGAATGCCCGCATTTGATAGTTTTTTCGGCATTTCTTTAACGTAGTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGga  <  1:162654/251‑1 (MQ=255)                                                                                                                                                                                          aCCACGACGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCAAAGACCAGCATATATGGGACGCGACGCAAAGTGTCCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCACGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGgcgc                >  1:82362/1‑228 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                            CCTTCCAGACCTGTTAAGCGAACTTCCTGGGCGCGAATTTCGCCATTGATACGGTTAGGGCGCGCCGTTTGAACTCGTTTTCCGCCTTTAATACCTTATTCCTCCAATTGTTTAAGACTGCGGCTGCGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGA  >  NC_000913/1800547‑1800973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑