Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,649	G→A	R169H (CGT→CAT)	mak →	fructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,649	0	G	A	100.0%	14.0 / NA	7	R169H (CGT→CAT)	mak	fructokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATT  >  NC_000913/410427‑410869                                                                                                                                                                                                                               |                                                                                                                                                                                                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATACGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCCGGTGAGTGAGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTACCTTGTTATTGc                                                                                                                                                                                                  >  1:38472/1‑251 (MQ=255)                        gCAAATGACGCTAACTGTCTGGCGGTTTCCGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAc                                                                                                                                                                           >  1:136067/1‑251 (MQ=255)                                                                          aGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGTGATTCGCGATGGATTATCGTCGTTTGAGCGGAc                                                                                                                         <  1:136267/251‑1 (MQ=255)                                                                                                gCNGTTNTNNTNNGCANGGGATACGGCGCGGGCGTGGCNNTCAATGGGCNGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACNGTGGATGGANNAANACGAANTNNNTNNNCGNGATGAAGGCCCTTGTTATTNCGNNAAACAANGTTGTATTGAAACCTTTATTTCGGGc                                                                                                                                                              <  2:38472/192‑1 (MQ=255)                                                                                                              naCGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTCCCTTGTTATTGCGGAAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACggggt                                                                                                                                                       >  1:19710/2‑183 (MQ=255)                                                                                                                                             nncnnctnnnngnnnnnnnncANNNGCACGGNANNGGAGTNGGGNAACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGATGAAGTCCCTTGTTATTGCGGTAAACAAGGTGGTATTGAAACCTTTATTTCGGGCACGNGATNCGCGNTGNATTATCGGCGCTGGANCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGNNANNNANCCGGTAGc                                                                  <  2:120775/219‑1 (MQ=255)                                                                                                                                                                     ngCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTCCCTTGTTAt                                                                                                                                                                                                     >  1:49267/2‑84 (MQ=255)                                                                                                                                                                                                 cGCTACCGTGGATGGACGAAGACGAACTGCATTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCAGTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATAtt  <  1:165860/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                             GGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATT  >  NC_000913/410427‑410869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑